ArticleScientific reports2016
TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Functional analysis of human induced pluripotent stem cell-derived cardiac tissue during endothelial cell network changes.Regenerative therapy · 2025Article
- Connexin 43 suppression enhances contractile force in human iPSC-derived cardiac tissues.Frontiers in bioengineering and biotechnology · 2025Article
- The Unique Roles of Ion Channels in Pluripotent Stem Cells in Response to Biological Stimuli.Biology · 2024Review
- Induction of cardiac alternans in human iPS-derived cardiomyocytes through β-adrenergic receptor stimulation.Physiological reports · 2024Article
- Low-adhesion culture selection for human iPS cell-derived cardiomyocytes.Scientific reports · 2024Article
- Modeling Reduced Contractility and Stiffness Using iPSC-Derived Cardiomyocytes Generated From Female Becker Muscular Dystrophy Carrier.JACC. Basic to translational science · 2023Article
- Development of appropriate fatty acid formulations to raise the contractility of constructed myocardial tissues.Regenerative therapy · 2022Article
- Bioartificial pulsatile cuffs fabricated from human induced pluripotent stem cell-derived cardiomyocytes using a pre-vascularization technique.NPJ Regenerative medicine · 2022Article
- Assessment of human bioengineered cardiac tissue function in hypoxic and re-oxygenized environments to understand functional recovery in heart failure.Regenerative therapy · 2021Article
- The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells.Cells · 2021Review
- An organic transistor matrix for multipoint intracellular action potential recording.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes.Scientific reports · 2021Article
- Evaluation of cell viability and metabolic activity of a 3D cultured human epidermal model using a dynamic autoradiographic technique with a PET radiopharmaceutical.Scientific reports · 2019Article
- CXCL4/PF4 is a predictive biomarker of cardiac differentiation potential of human induced pluripotent stem cells.Scientific reports · 2019Article
- Contractile force measurement of human induced pluripotent stem cell-derived cardiac cell sheet-tissue.PloS one · 2018Article
- Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue.Scientific reports · 2017Article
- Tubular Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells Generate Pulse Pressure In Vivo.Scientific reports · 2017Article
- Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of Its Synchronous Beatings by Optical Coherence Tomography.BioMed research international · 2017Article
- Novel surface-enhanced Raman scattering-based assays for ultra-sensitive detection of human pluripotent stem cells.Biomaterials · 2016Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, we show for the first time that TRPV-1 activation through transient culture at 42 °C in combination with agonists is a simple and useful strategy to eliminate iPS cells from bioengineered cardiac cell sheet tissues. When human iPS cells were cultured at 42 °C, almost all cells disappeared by 48 hours through apoptosis. However, iPS cell-derived cardiomyocytes and fibroblasts maintained transcriptional and protein expression levels, and cardiac cell sheets were fabricated after reducing the temperature. TRPV-1 expression in iPS cells was upregulated at 42 °C, and iPS cell death at 42 °C was TRPV-1-dependent. Furthermore, TRPV-1 activation through thermal or agonist treatment eliminated iPS cells in cardiac tissues for a final concentration of 0.4% iPS cell contamination. These findings suggest that the difference in tolerance to TRPV-1 activation between iPS cells and iPS cell-derived cardiac cells could be exploited to eliminate remaining iPS cells in bioengineered cell sheet tissues, which will further reduce the risk of tumour formation.
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