ArticleJournal of biomedical materials research. Part B, Applied biomaterials2017
Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells.
Article in Journal of biomedical materials research. Part B, Applied biomaterials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- AI driven 3D subcellular RPE map discovers cell state transitions in establishment of apical-basal polarity.NPJ artificial intelligence · 2026Article
- Role of Platelet-Rich Plasma Gel in Promoting Wound Healing Based on Medical Images of Wounds.Contrast media & molecular imaging · 2022Article
- Mechanistic role of perfusion culture on bone regeneration.Journal of biosciences · 2019Review
- Machine learning metrology of cell confinement in melt electrowritten three-dimensional biomaterial substrates.Microsystems & nanoengineering · 2019Article
- Effect of the scaffold microenvironment on cell polarizability and capacitance determined by probabilistic computations.Biomedical materials (Bristol, England) · 2018Article
- Enhanced Differentiation of Human Preosteoblasts on Electrospun Blend Fiber Mats of Polydioxanone and Anionic Sulfated Polysaccharides.ACS biomaterials science & engineering · 2017Article
- A Review of the Fundamental Principles and Applications of Solution Blow Spinning.ACS applied materials & interfaces · 2016Article
- Recent advances in nano scaffolds for bone repair.Bone research · 2016Review
- Effects of nanofibers on mesenchymal stem cells: environmental factors affecting cell adhesion and osteogenic differentiation and their mechanisms.Journal of Zhejiang University. Science. BReview
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Authors and funding
4 authors.
Funding
Abstract
Recent work demonstrates that osteoprogenitor cell culture on nanofiber scaffolds can promote differentiation. This response may be driven by changes in cell morphology caused by the three-dimensional (3D) structure of nanofibers. We hypothesized that nanofiber effects on cell behavior may be mediated by changes in organelle structure and function. To test this hypothesis, human bone marrow stromal cells (hBMSCs) were cultured on poly(ε-caprolactone) (PCL) nanofibers scaffolds and on PCL flat spuncoat films. After 1 day-culture, hBMSCs were stained for actin, nucleus, mitochondria, and peroxisomes, and then imaged using 3D confocal microscopy. Imaging revealed that the hBMSC cell body (actin) and peroxisomal volume were reduced during culture on nanofibers. In addition, the nucleus and peroxisomes occupied a larger fraction of cell volume during culture on nanofibers than on films, suggesting enhancement of the nuclear and peroxisomal functional capacity. Organelles adopted morphologies with greater 3D-character on nanofibers, where the Z-Depth (a measure of cell thickness) was increased. Comparisons of organelle positions indicated that the nucleus, mitochondria, and peroxisomes were closer to the cell center (actin) for nanofibers, suggesting that nanofiber culture induced active organelle positioning. The smaller cell volume and more centralized organelle positioning would reduce the energy cost of inter-organelle vesicular transport during culture on nanofibers. Finally, hBMSC bioassay measurements (DNA, peroxidase, bioreductive potential, lactate, and adenosine triphosphate (ATP)) indicated that peroxidase activity may be enhanced during nanofiber culture. These results demonstrate that culture of hBMSCs on nanofibers caused changes in organelle structure and positioning, which may affect organelle functional capacity and transport. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. J Biomed Mater Res Part B: Appl Biomater, 2016. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 989-1001, 2017.
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