Evidence map›Paper›PMID 26888440›Full record

ArticleBiomedical microdevices2016

A dual wedge microneedle for sampling of perilymph solution via round window membrane.

Hirobumi Watanabe, Luis Cardoso, Anil K Lalwani, Jeffrey W Kysar

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical microdevices, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
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  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Microtechnologies for inner ear drug delivery.Current opinion in otolaryngology & head and neck surgery · 2020
    Review
  11. Inner ear delivery: Challenges and opportunities.Laryngoscope investigative otolaryngology · 2020
    Review
  12. Anatomical and Functional Consequences of Microneedle Perforation of Round Window Membrane.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2020
    Article
  13. 3D-Printed Microneedles Create Precise Perforations in Human Round Window Membrane in Situ.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2020
    Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Hirobumi WatanabeDepartment of Mechanical Engineering, Columbia University, 220 Mudd Building 500 West 120th Street, New York, NY, 10027, USA. hw2420@columbia.edu.
Luis CardosoDepartment of Biomedical Engineering, The City College of The University of New York, New York, NY, 10031, USA.
Anil K LalwaniDepartment of Otolaryngology - Head and Neck Surgery, Columbia University College of Physicians and Surgeons, New York, NY, 10032, USA.
Jeffrey W KysarDepartment of Mechanical Engineering, Columbia University, 220 Mudd Building 500 West 120th Street, New York, NY, 10027, USA.
Columbia University · USCity College of New York · US

Funding

Intracochlear Delivery of Therapeutics Across RWM via Microneedle ArrayR01DC014547 · NIDCD · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI KYSAR, JEFFREY W., LALWANI, ANIL K · 2015 to 2019
$2.1M
NIDCD NIH HHS R01 DC014547NIDCD NIH HHS R01DC014547
6 · The paper itself

Abstract

Precision medicine for inner-ear disease is hampered by the absence of a methodology to sample inner-ear fluid atraumatically. The round window membrane (RWM) is an attractive portal for accessing cochlear fluids as it heals spontaneously. In this study, we report on the development of a microneedle for perilymph sampling that minimizes the size of RWM perforation, facilitates quick aspiration, and provides precise volume control. Here, considering the mechanical anisotropy of the RWM and hydrodynamics through a microneedle, a 31G stainless steel pipe was machined into wedge-shaped design via electrical discharge machining. The sharpness of the needle was evaluated via a surface profilometer. Guinea pig RWM was penetrated in vitro, and 1 μL of perilymph was sampled and analyzed via UV-vis spectroscopy. The prototype wedge shaped needle was successfully fabricated with the tip curvature of 4.5 μm and the surface roughness of 3.66 μm in root mean square. The needle created oval perforation with minor and major diameter of 143 and 344 μm (n = 6). The sampling duration and standard deviation of aspirated volume were 3 s and 6.8 % respectively. The protein concentration was 1.74 mg/mL. The prototype needle facilitated precise perforation of RWMs and rapid aspiration of cochlear fluid with precise volume control. The needle design is promising and requires testing in human cadaveric temporal bone and further optimization to become clinically viable.

Indexed as

NeedlesPerilymphAnimalsCochleaEquipment DesignGuinea PigsHumansMicrotechnologyParacentesisSolutionsTime FactorsTympanic MembraneSolutionsInner ear diseaseMicroneedleMinimally invasive samplingPersonalized medicine

Identifiers

PMID26888440
PMCPMC5574191
OpenAlexW2277973125

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.