ArticleProceedings of the National Academy of Sciences of the United States of America2016
ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation of auxin transporter recycling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Light and Shadows: Insights from Large-Scale Visual Screens for Arabidopsis Leaf Morphology Mutants.International journal of molecular sciences · 2025Review
- Sequestration of DBR1 to stress granules promotes lariat intronic RNAs accumulation for heat-stress tolerance.Nature communications · 2024Article
- Time-Dependent Proteomic Signatures Associated with Embryogenic Callus Induction inPlants (Basel, Switzerland) · 2023Article
- Absence of SICKLE triggers programed cell death by disturbing alternative splicing and decay of mRNAs.Plant physiology · 2023Article
- The Chromatin Remodeling FactorPlants (Basel, Switzerland) · 2023Article
- Phosphorylation status of Bβ subunit acts as a switch to regulate the function of phosphatase PP2A in ethylene-mediated root growth inhibition.The New phytologist · 2022Article
- SICKLE modulates lateral root development by promoting degradation of lariat intronic RNA.Plant physiology · 2022Article
- The Protein Phosphatase PP2A Plays Multiple Roles in Plant Development by Regulation of Vesicle Traffic-Facts and Questions.International journal of molecular sciences · 2021Review
- Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective.Frontiers in plant science · 2021Article
- Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.Current biology : CB · 2020Article
- Root Growth Adaptation is Mediated by PYLs ABA Receptor-PP2A Protein Phosphatase Complex.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Remove, Recycle, Degrade: Regulating Plasma Membrane Protein Accumulation.The Plant cell · 2019Review
- Exogenous Application of Phytohormones Promotes Growth and Regulates Expression of Wood Formation-Related Genes inInternational journal of molecular sciences · 2019Article
- PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism.Scientific reports · 2018Article
- Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling: Strigolactones in the Spotlight.Frontiers in plant science · 2018Article
- Arabidopsis choline transporter-like 1 (CTL1) regulates secretory trafficking of auxin transporters to control seedling growth.PLoS biology · 2017Article
- The Arabidopsis sickle Mutant Exhibits Altered Circadian Clock Responses to Cool Temperatures and Temperature-Dependent Alternative Splicing.The Plant cell · 2016Article
- Regulation of polar auxin transport by protein and lipid kinases.Journal of experimental botany · 2016Review
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Authors and funding
15 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The shaping of organs in plants depends on the intercellular flow of the phytohormone auxin, of which the directional signaling is determined by the polar subcellular localization of PIN-FORMED (PIN) auxin transport proteins. Phosphorylation dynamics of PIN proteins are affected by the protein phosphatase 2A (PP2A) and the PINOID kinase, which act antagonistically to mediate their apical-basal polar delivery. Here, we identified the ROTUNDA3 (RON3) protein as a regulator of the PP2A phosphatase activity in Arabidopsis thaliana. The RON3 gene was map-based cloned starting from the ron3-1 leaf mutant and found to be a unique, plant-specific gene coding for a protein with high and dispersed proline content. The ron3-1 and ron3-2 mutant phenotypes [i.e., reduced apical dominance, primary root length, lateral root emergence, and growth; increased ectopic stages II, IV, and V lateral root primordia; decreased auxin maxima in indole-3-acetic acid (IAA)-treated root apical meristems; hypergravitropic root growth and response; increased IAA levels in shoot apices; and reduced auxin accumulation in root meristems] support a role for RON3 in auxin biology. The affinity-purified PP2A complex with RON3 as bait suggested that RON3 might act in PIN transporter trafficking. Indeed, pharmacological interference with vesicle trafficking processes revealed that single ron3-2 and double ron3-2 rcn1 mutants have altered PIN polarity and endocytosis in specific cells. Our data indicate that RON3 contributes to auxin-mediated development by playing a role in PIN recycling and polarity establishment through regulation of the PP2A complex activity.
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