Evidence map›Paper›PMID 26887971›Full record

ArticleArchives of virology2016

One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus.

Marta Budziszewska, Przemysław Wieczorek, Aleksandra Obrępalska-Stęplowska

Abstract read
In one paragraph

Article in Archives of virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Marta BudziszewskaInterdepartmental Laboratory of Molecular Biology, Institute of Plant Protection-National Research Institute, Władysława Wegorka 20, 60-318, Poznan, Poland.
Przemysław WieczorekInterdepartmental Laboratory of Molecular Biology, Institute of Plant Protection-National Research Institute, Władysława Wegorka 20, 60-318, Poznan, Poland.
Aleksandra Obrępalska-StęplowskaInterdepartmental Laboratory of Molecular Biology, Institute of Plant Protection-National Research Institute, Władysława Wegorka 20, 60-318, Poznan, Poland. olaob@o2.pl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

'Torrado' disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive.

Indexed as

Base SequenceMolecular Sequence DataNucleic Acid Amplification TechniquesPicornaviridaePicornaviridae InfectionsRNA, ViralSequence AlignmentSolanum lycopersicumRNA, ViralDiagnostic protocolPlant virus detectionRT-LAMPTorradovirusVirus diagnostics

Identifiers

PMID26887971
PMCPMC4839060

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.