ArticleJournal of materials science. Materials in medicine2016
Impact of decellularization on porcine myocardium as scaffold for tissue engineered heart tissue.
Article in Journal of materials science. Materials in medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Biological tissue engineering for Fontan failure using decellularized extracellular matrix: a systematic review.Journal of translational medicine · 2026Pooled it
- Evaluation of Different Decellularization Protocols for Obtaining and Characterizing Canine Cardiac Extracellular Matrix.Biomedicines · 2024Article
- Atomic force microscopy for characterization of decellularized extracellular matrix (dECM) based materials.Science and technology of advanced materials · 2024Review
- Recent advances in soluble decellularized extracellular matrix for heart tissue engineering and organ modeling.Journal of biomaterials applications · 2023Review
- Review
- Three-Dimensional Bioprinting of Decellularized Extracellular Matrix-Based Bioinks for Tissue Engineering.Molecules (Basel, Switzerland) · 2022Review
- Optimization of Complete Rat Heart Decellularization Using Artificial Neural Networks.Micromachines · 2022Article
- Development of a new decellularization protocol for the whole porcine heart.Journal of clinical and translational research · 2021Article
- Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks.Molecules (Basel, Switzerland) · 2020Review
- Cardiac tissue-derived extracellular matrix scaffolds for myocardial repair: advantages and challenges.Regenerative biomaterials · 2019Review
- Trans-differentiation of human adipose-derived mesenchymal stem cells into cardiomyocyte-like cells on decellularized bovine myocardial extracellular matrix-based films.Journal of materials science. Materials in medicine · 2018Article
- Magnesium Presence Prevents Removal of Antigenic Nuclear-Associated Proteins from Bovine Pericardium for Heart Valve Engineering.Tissue engineering. Part A · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Decellularized myocardium has been proposed to construct tissue engineered heart tissue, providing the advantage of natural extracellular architecture. Various decellularization protocols have been developed, but the impact of individual decellularization reagent in the protocol remains unclear. The aim of this study is to evaluate the structural impact of three commonly used decellularization reagents on the porcine myocardium. We decellularized porcine heart tissue with trypsin, Triton X-100 or SDS, and analyzed the morphological characteristics of the remaining tissue by SEM, AFM and two-photon LSM. We further recellularized the scaffold with rat myocardial fibroblasts and cardiomyocytes separately. According to the H&E staining and DNA quantification, SDS decellularized more efficiently in comparison to the other two reagents. Moreover, we found distinct surface microarchitecture differences among groups. The changed structure of tissue might result in varied proliferation myocardial fibroblasts and biophysical performance of the engineered heart tissue. This study demonstrated that the microstructure of decellularized porcine heart tissue vary with decellularization agents. Compared to trypsin and Triton X-100, SDS not only decellularized more efficiently but also preserved the biocompatible microstructure of ECM for recellularization.
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Registered trials
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