ArticleJournal of materials science. Materials in medicine2016
The response of osteoblastic MC3T3-E1 cells to micro- and nano-textured, hydrophilic and bioactive titanium surfaces.
Article in Journal of materials science. Materials in medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Surface Modifications of cpTi and Ti-6Al‑4V: The Synergistic Potential of PEO-CaP for Optimized Dental Implant Performance.ACS omega · 2026Article
- Topography-based implants for bone regeneration: Design, biological mechanism, and therapeutics.Materials today. Bio · 2025Review
- Preparation of human primary macrophages to study the polarization from monocyte-derived macrophages to pro- or anti-inflammatory macrophages at biomaterial interfaceJournal of dental sciences · 2023Article
- Functional engineering strategies of 3D printed implants for hard tissue replacement.Regenerative biomaterials · 2023Review
- Osteoblast Attachment on Titanium Coated with Hydroxyapatite by Atomic Layer Deposition.Biomolecules · 2022Article
- Effect of microtopography on osseointegration of implantable biomaterials and its modification strategies.Frontiers in bioengineering and biotechnology · 2022Review
- Decreased expression of miR-195 mediated by hypermethylation promotes osteosarcoma.Open medicine (Warsaw, Poland) · 2022Article
- Effect of a Nanostructured Titanium Surface on Gingival Cell Adhesion, Viability and Properties againstMaterials (Basel, Switzerland) · 2021Article
- Osteosphere Model to Evaluate Cell-Surface Interactions of Implantable Biomaterials.Materials (Basel, Switzerland) · 2021Article
- Construction of Complex Structures Containing Micro-Pits and Nano-Pits on the Surface of Titanium for Cytocompatibility Improvement.Materials (Basel, Switzerland) · 2019Article
- Strontium inhibits osteoclastogenesis by enhancing LRP6 and β-catenin-mediated OPG targeted by miR-181d-5p.Journal of cell communication and signaling · 2019Article
- In Vitro Assessment of Early Bacterial Activity on Micro/Nanostructured Ti6Al4V Surfaces.Molecules (Basel, Switzerland) · 2017Article
- Improved in vitro angiogenic behavior on anodized titanium dioxide nanotubes.Journal of nanobiotechnology · 2017Article
- ClC-3 chloride channel mediates the role of parathyroid hormone [1-34] on osteogenic differentiation of osteoblasts.PloS one · 2017Article
- A superhydrophilic titanium implant functionalized by ozone gas modulates bone marrow cell and macrophage responses.Journal of materials science. Materials in medicine · 2016Article
- Tardive Dyskinesia, Oral Parafunction, and Implant-Supported Rehabilitation.Case reports in dentistry · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present work was to investigate the morphology and activity of the murine osteoblastic cell line MC3T3 on control smooth (Machined), commercially available rough (ZT) titanium discs, and on titanium samples obtained by modifying the ZT treatment protocol, and herein labelled as ZTF, ZTM and ZTFM. Cells were evaluated at SEM and immunofluorescence for morphology and cell-to-cell interactions and by MTT assay and real time PCR for cell growth and function. Microscopy showed that ZT modified protocols could differently affect cell shape and distribution. All the tested surfaces showed good biocompatibility by viability assay. However, cells on smoother surfaces appeared to express higher levels of transcript for Collagen 1a1, the main component of extracellular matrix, by real time PCR. Expression of the early differentiation marker Alkaline Phosphatase was higher on ZTF surfaces and ZTM enhanced the expression of later osteoblastic markers Osteoprotegerin and Osteocalcin. Noteworthy, the expression of Connexin 43, a component of cell-to-cell contacts and hemichannels, followed a similar pattern to differentiation marker genes and was higher in cells on ZTM surfaces, consistently with the microscopic observation of cell clusters. Taken together, this data showed that ZTF and ZTM treatment protocols appeared to improve the basal sand-blasting/acid-etching ZT procedure with ZTM surfaces promoting the most mature stage of differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.