Evidence map›Paper›PMID 26885278›Full record

ArticleAmerican journal of translational research2015

Characterization of arginine kinase, anovel allergen of dermatophagoides farinae (Der f 20).

Peng Xing, Haiqiong Yu, Meng Li, Xiaojun Xiao, Congli Jiang, Lihua Mo, Min Zhang, Pingchang Yang, Zhigang Liu

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in American journal of translational research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

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  8. Expression inAllergy, asthma & immunology research · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Peng XingOtolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine3688 Nanhai Blvd., Shenzhen 518060, China.
Haiqiong YuThe State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
Meng LiThe State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
Xiaojun XiaoThe State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
Congli JiangThe State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
Lihua MoThe State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
Min ZhangFirst Affiliated Hospital of Shenzhen University, Shenzhen Second Peopl's Hospital Shenzhen, Guangdong 518035, China.
Pingchang YangOtolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine3688 Nanhai Blvd., Shenzhen 518060, China.
Zhigang LiuOtolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine3688 Nanhai Blvd., Shenzhen 518060, China.
Shenzhen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo characterize a novel allergen, the Dermatophagoides farinae-derived arginine kinase (Der f 20).

methodsThe protein of Der f 20 was synthesized by genetic engineering approaches. The allergenicity of Der f 20 was tested by enzyme-linked immunosorbent assay and an airway allergy mouse model.

resultsThe Der f 20 gene was cloned andpresented in the Gene Bank with an accession number of AAP57094. The Der f 20 is an arginine kinase (AK), whichshowed a close relationship with D. pteronyssinus AK and Aleuroglyphusovatus AK. Western-blot and ELISA studies showed the IgE binding capacity of Der f 20 was 66.7% in the sera from 6 dust mite allergic patients. Immune inhibition assayresults showed the IgE cross-reactivity between Der f 20 and DME (Dust mite extract). Positive responses to Der f 20 were 41.2% as shown by skin prick tests in 17 DME-allergic patients. In vitro experimental results showed that Der f 20 induced Th2 cell differentiation and the expression of T cell Ig mucin domain molecule-4 (TIM4) in DCs. Conclusions; The Der f 20 protein is a novel subtype of thedust mite allergen.

Indexed as

allergyArginine kinaseDer f 20dermatophagoidesfarinaeprokaryotic expression

Identifiers

PMID26885278
PMCPMC4731678
OpenAlexW2274610594

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.