ArticleAmerican journal of translational research2015
c9, t11- conjugated linoleic acid induces HCC cell apoptosis and correlation with PPAR-γ signaling pathway.
Article in American journal of translational research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Long non‑coding RNA HOTTIP promotes hepatocellular carcinoma tumorigenesis and development: A comprehensive investigation based on bioinformatics, qRT‑PCR and meta‑analysis of 393 cases.International journal of oncology · 2017Pooled it
- Conjugated Linoleic Acid in Cancer Therapy.Current drug delivery · 2025Review
- Uncovering the Mechanisms of Active Components from Toad Venom against Hepatocellular Carcinoma Using Untargeted Metabolomics.Molecules (Basel, Switzerland) · 2022Article
- Alpha-linolenic acid inhibits hepatocellular carcinoma cell growth through Farnesoid X receptor/β-catenin signaling pathway.Nutrition & metabolism · 2022Article
- Unsaturated fatty acids as a co-therapeutic agents in cancer treatment.Molecular biology reports · 2021Review
- Transferrin-Conjugated Erianin-Loaded Liposomes Suppress the Growth of Liver Cancer by Modulating Oxidative Stress.Frontiers in oncology · 2021Article
- Sheep and Goats Respond Differently to Feeding Strategies Directed to Improve the Fatty Acid Profile of Milk Fat.Animals : an open access journal from MDPI · 2020Review
- The influence of conjugated linoleic acid on the expression of peroxisome proliferator-activated receptor-γ and selected apoptotic genes in non-small cell lung cancer.Molecular and cellular biochemistry · 2020Article
- Tracking the important role ofOncology letters · 2020Article
- [Research advances in the pathogenesis of non-alcoholic fatty liver disease-associated liver cancer].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2017Review
- Down-regulation of MiR-365 as a novel indicator to assess the progression and metastasis of hepatocellular carcinoma.International journal of clinical and experimental pathology · 2017Article
- Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells.PPAR research · 2017Article
- Peroxisome proliferator-activated receptor-γ agonist troglitazone suppresses transforming growth factor-β1 signalling through miR-92b upregulation-inhibited Axl expression in human keloid fibroblasts in vitro.American journal of translational research · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveCis9, trans11 conjugated linoleic acid (c9, t11-CLA.) is one of the most important isomers of conjugated linoleic acid, which have a strong anti-tumor effects. Based on previous studies, we further explored the molecular mechanism of inducing cells apoptosis in human hepatocellular carcinoma cell line HepG2 and Hep3B.
methodsCell Counting Kit 8 (CCK-8) assay was used to investigate the effects of c9, t11-CLA on cell viability and cell proliferation ability; The effects of c9, t11-CLA on cell apoptosis was analyzed by DNA ladder assay, immuno-fluorescence and flow cytometry, respectively. Apoptotic related gene (Bcl-2, Bcl-XL, Bcl-w, Mcl-1, Bax, Bak, Bad, Bid and Bim), PPAR family member (PPAR-α, PPAR-β and PPAR-γ), and Cox2 mRNA and protein expression were analyzed by RT-PCR and western blotting. ELISA assay was used to detect the content of Caspase-3.
resultsOur data were confirmed that c9, t11-CLA could inhibit the HCC cells proliferation ability and decrease the cells viability. RT-PCR and western blotting assay verified that c9, t11-CLA obviously increased the transcription and protein expression levels of PPAR-γ. The synchronism and correlation between PPAR-γ and apoptotic proteins Bcl-2, Bax and Caspase-3 were found with a dose- and time-dependent manner. PPAR-γ inhibitor GW9662 and activator Rosilitazone were further verified that there was cooperative relation between them.
conclusionIn our study, we first report that c9, t11-CLA induces apoptosis in HCC cells by activation of PPARγ-Bcl-2-Caspase-3 signal pathway. These results indicated that c9, t11-CLA will be useful for clinic therapy of anti-tumor and as a new regulator of PPAR-γ in the future.
Indexed as
Identifiers
26885272PMC4731672W2412287511What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.