ArticleInternational journal of clinical and experimental medicine2015
Roles of mycobacterium tuberculosis ESAT-6 in the development of renal injury.
Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- A crucial role of miR-155 in the pathomechanism of acute kidney injury.Frontiers in pharmacology · 2025Review
- SEVERITY OF TB CLASSIFIED BY MODIFIED BANDIM TB SCORING ASSOCIATES WITH THE SPECIFIC SEQUENCE OF ESXA GENES IN MDR-TB PATIENTS.African journal of infectious diseases · 2020Article
- An Inflammatory Story: Antibodies in Tuberculosis Comorbidities.Frontiers in immunology · 2019Review
- Tuberculosis-associated IgA nephropathy.The Journal of international medical research · 2018Review
- Role of ESAT-6 in renal injury by regulating microRNA-155 expression via TLR4/MyD88 signaling pathway in mice withBioscience reports · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe present study was designed to evaluate the role of mycobacterium tuberculosis early secretory antigen target-6 (MtbESAT-6) in the development of renal injury.
methodsPET42a (+) ESAT6 prokaryotic expression plasmid was constructed and the purified ESAT6 protein without endotoxin was obtained. Sixty healthy, clean, male Kunming mice were randomly divided into two groups: the experimental group (n = 30) and the control group (n = 30). Each mouse in the experimental group were injected with 0.5 ml ESAT-6 protein, and each mouse in the control group were injected with 0.5 ml sterile saline on the tail vein. Blood, urine and kidney tissues were collected. Serum creatinine (Scr), blood urea nitrogen (BUN), and urinary creatinine (Cr) were determined by HITACHI 7150 automatic biochemical analyzer and creatinine clearance rate (Ccr) was calculated. Renal tissues were conducted for hematoxylin-eosin (HE) staining and pathological scores of renal injury were recorded under the light microscope.
resultsUsing MTB H37Ra strains genome DNA as template, the ESAT6 gene amplified by Hieff Pfu DNA Polymerase using polymerase chain reaction (PCR) technique was consistent with the expected size. PET42a (+) ESAT6 vector plasmid was successfully obtained and ESAT6 recombinant protein was successfully expressed with the protein concentration of 1.69 mg/ml. BUN and Scr in the experimental group were gradually increased, Ccr was gradually decreased, and the pathological score of renal injury increased gradually, and all of which were significantly higher than that in the control group after the experiment of 12 h, 24 h and 48 h (all P < 0.05).
conclusionMtbESAT-6 might contribute to the development of renal injury.
Indexed as
Identifiers
26885168PMC4724014W2287971612What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.