ArticleInternational journal of clinical and experimental medicine2015
Effects of down-regulation of clusterin by small interference RNA on human acute myeloid leukemia cells.
Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 11 citations in OpenAlex.
- Clusterin mediates hydroquinone-induced cytotoxic responses in HL-60 differentiated cells.Scientific reports · 2024Article
- Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells.BMC cancer · 2020Article
- A New Approach for the Diagnosis of Myelodysplastic Syndrome Subtypes Based on Protein Interaction Analysis.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
AIMS AND
backgroundUp-regulation of clusterin is associated with the survival and progression of various malignancies, and down-regulation of clusterin promotes apoptosis and inhibits invasion. The aim of this study was to explore the effect of clusterin small interference RNA (siRNA) on the proliferation, apoptosis and invasion of HL-60 acute myeloid leukemia (AML) cells.
methodssiRNA transfection was performed using Lipofectamine™2000 reagent. Relative protein expressions were quantified by Western blot. Trypan blue assay was performed to assess tumor cell proliferation after siRNA transfection. The cytotoxic effect of clusterin siRNA on leukemic cells was measured using MTT assay. Apoptosis was detected using fluorescence microscopy assay. Migration and invasion was detected after clusterin was silenced.
resultsClusterin siRNA clearly lowered clusterin protein levels in a time- dependent manner, leading to marked inhibition of cell survival, proliferation and invasion. Furthermore, clusterin down-regulation significantly enhanced the extent of HL-60 apoptotic cells.
conclusionsOur results suggest that the down-regulation of clusterin by siRNA can effectively trigger apoptosis and inhibit the proliferation and invasion of leukemic cells. Therefore, clusterin siRNA may be a potent adjuvant in AML therapy.
Indexed as
Identifiers
26885020PMC4723865W2440267275What OpenQuestion holds
Registered trials
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