ArticleInternational journal of clinical and experimental medicine2015
Effects of microRNA-139 on myocardial cell injury induced by oxidative stress.
Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The trial behind it
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Who cites it
4 citing papers in PubMed, 8 citations in OpenAlex.
- Inflammation and Nitro-oxidative Stress as Drivers of Endocannabinoid System Aberrations in Mood Disorders and Schizophrenia.Molecular neurobiology · 2022Review
- Differential expressions of miR-223, miR-424, miR-145, miR-200c, miR-139 in experimental rat chronic pancreatitis model and their relationship between oxidative stress, endoplasmic reticulum stress, and apoptosis.Iranian journal of basic medical sciences · 2021Article
- MicroRNA expression profiling and bioinformatics analysis of dysregulated microRNAs in obstructive sleep apnea patients.Medicine · 2017Observational
- Mangiferin: a natural miracle bioactive compound against lifestyle related disorders.Lipids in health and disease · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study aims to explore the effects of miR-139 on myocardial cell injury induced by oxidative stress and its mechanisms.
methodsH9c2 cells were used in this study. They were divided into control group, H2O2 group, H2O2+miR-139-5-p NC group and H2O2+miR-139-5-p mimics group. Cell activity was detected by MTT method. ROS level was detected by DCFH-DA probe method. MDA and SOD levels and Caspase 3 activity were detected by spectrophotometry. The cell apoptosis was detected by Hoechst 33342 and Annexin V-FITC/PI staining methods. The expression levels of AKT, GSK-3β, Bax and Bcl-2 were determined by Western blotting methods.
resultsIt showed that the activity of H9c2 cells decreased with the increase of the dose of H2O2. The activity of miR-139-5-p in H9c2 cells decreased after treatment of H2O2 for 6 h (P<0.01). Compared with control group, cell activity in H2O2 group and H2O2+miR-139-5-p NC group decreased (P<0.01), ROS fluorescence intensity increased (P<0.01), MDA content increased (P<0.01), SOD content decreased (P<0.01), apoptosis degree, Caspase 3 activity and Bax levels increased (P<0.01), Bcl-2, AKT and GSK-3β decreased (P<0.01). However, they were opposite in H2O2+miR-139-5-p mimics compared with H2O2 group and H2O2+miR-139-5-p NC group.
conclusionsmiR-139-5-p expressed low in oxidative stress of H9c2 cells induced by H2O2 and the oxidative stress injury could be inhibited after increasing the expression of miR-139-5-p, which could be related with the elimination of intracellular oxidative stress products and the resistance to apoptosis through AKT/GSK-3β signaling pathway.
Indexed as
Identifiers
26884911PMC4723756W2441153412What OpenQuestion holds
Registered trials
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