Evidence map›Paper›PMID 26884817›Full record

ReviewJournal of chemical biology2015

FluoroNanogold: an important probe for correlative microscopy.

Toshihiro Takizawa, Richard D Powell, James F Hainfeld, John M Robinson

Open access · bronzeAbstract readReview
In one paragraph

Review in Journal of chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024
    Review
  3. Article
  4. Nanoscale advances · 2023
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Toshihiro TakizawaDepartment of Molecular Anatomy, Nippon Medical School, Tokyo, Japan.
Richard D PowellNanoprobes, Incorporated, 95 Horseblock Road, Unit 1, Yaphank, NY 11980-9710 USA.
James F HainfeldNanoprobes, Incorporated, 95 Horseblock Road, Unit 1, Yaphank, NY 11980-9710 USA.
John M RobinsonDepartment of Physiology and Cell Biology, Ohio State University, Columbus, OH 43210 USA.
Nanoprobes (United States) · USNippon Medical School · JPThe Ohio State University · US

Funding

Plasma Membrane Repair Mechanisms in Placental TrophoblastsR01HD058084 · NICHD · OHIO STATE UNIVERSITY · PI ROBINSON, JOHN MITCHELL · 2010 to 2014
$1.5M
NICHD NIH HHS R01 HD058084
6 · The paper itself

Abstract

Correlative microscopy is a powerful imaging approach that refers to observing the same exact structures within a specimen by two or more imaging modalities. In biological samples, this typically means examining the same sub-cellular feature with different imaging methods. Correlative microscopy is not restricted to the domains of fluorescence microscopy and electron microscopy; however, currently, most correlative microscopy studies combine these two methods, and in this review, we will focus on the use of fluorescence and electron microscopy. Successful correlative fluorescence and electron microscopy requires probes, or reporter systems, from which useful information can be obtained with each of the imaging modalities employed. The bi-functional immunolabeling reagent, FluoroNanogold, is one such probe that provides robust signals in both fluorescence and electron microscopy. It consists of a gold cluster compound that is visualized by electron microscopy and a covalently attached fluorophore that is visualized by fluorescence microscopy. FluoroNanogold has been an extremely useful labeling reagent in correlative microscopy studies. In this report, we present an overview of research using this unique probe.

Indexed as

Correlative microscopyFluoroNanogoldImmunocytochemistryNanogoldUltrathin cryosections

Identifiers

PMID26884817
PMCPMC4744603
OpenAlexW1191007519

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.