ArticleThe journal of venomous animals and toxins including tropical diseases2016
Anticancer properties of phospholipase A2 from Daboia siamensis venom on human skin melanoma cells.
Article in The journal of venomous animals and toxins including tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Biochemical characterization and activity profiling of recombinant phospholipase A2 from Hemiscorpius lepturus expressed in E. coli with in vivo antibody response.Scientific reports · 2025Article
- Functional Characterization and Anti-Tumor Effect of a Novel Group II Secreted Phospholipase AMolecules (Basel, Switzerland) · 2023Article
- Protective action of ultrasound-guided electrolysis technique on the muscle damage induced by notexin in rats.PloS one · 2022Article
- Article
- Cytotoxicity of snake venom enzymatic toxins: phospholipase A2 and l-amino acid oxidase.Biochemical Society transactions · 2020Review
- Protective Effects of Foam Rolling against Inflammation and Notexin Induced Muscle Damage in Rats.International journal of medical sciences · 2020Article
- Article
- Cytotoxic and pro-apoptotic action of MjTX-I, a phospholipase A2 isolated fromThe journal of venomous animals and toxins including tropical diseases · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhospholipase A2 (PLA2) is a major component of the Daboia siamensis venom, which is able to hydrolyse the membrane of various cells. For this reason, the activity of PLA2 was investigated regarding its pharmaceutical properties. This study was conducted to explore the pharmacological properties of a PLA2 from Daboia siamensis (dssPLA2) venom on human skin melanoma cell line (SK-MEL-28).
methodsdssPLA2 was isolated by ion exchange and gel filtration columns. Various concentrations of dssPLA2 were investigated for cytotoxic activity and inhibition of migration on SK-MEL-28 cells. Cell death analysis, mRNA expression levels of Notch I-III and BRAF V600E genes were also determined.
resultsdssPLA2 exhibited cytotoxicity on SK-MEL-28 for 24 and 72 h as compared with untreated cells. However, it had no toxic effects on CCD-1064sk cells under the same conditions. dssPLA2 (0.25 and 0.5 μg/mL) induced 17.16 and 30.60 % of apoptosis, while activated 6.53 and 7.05 % of necrotic cells. dssPLA2 at 0.25, 0.5, 1 and 2 μg/mL could inhibit migration on SK-MEL-28 cells for 24 h by 31.06, 41.66, 50 and 68.75 %, respectively. The action of dssPLA2 significantly reduced the levels of Notch I and BRAF V600E genes expression on SK-MEL-28 cells compared with untreated cells at 72 h.
conclusionsThis study indicates that dssPLA2 had potential effects of apoptosis, necrosis, cytotoxicity and inhibition of migration on SK-MEL-28 cells. dssPLA2 could possibly be a selective agent that targets cancer cells without affecting normal cells.
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