ArticleDevelopment (Cambridge, England)2016
Molecular model for force production and transmission during vertebrate gastrulation.
Article in Development (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 68 citations in OpenAlex.
- Quantifying convergent extension: Shih and Keller's quintessential work in developmental cell biology.Developmental biology · 2025Article
- PCP-dependent polarized mechanics in the cortex of individual cells during convergent extension.Developmental biology · 2025Article
- The first embryo, the origin of cancer and animal phylogeny. V. Cancer stem cells as the unifying biomechanical principle between embryology and oncology.Mechanobiology in medicine · 2025Review
- Inferring active and passive mechanical drivers of epithelial convergent extension.bioRxiv : the preprint server for biology · 2025Article
- Distinct mechanisms regulate ventricular and atrial chamber wall formation.Nature communications · 2024Article
- PCP and Septins govern the polarized organization of the actin cytoskeleton during convergent extension.Current biology : CB · 2024Article
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- Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Dorsal lip maturation and initial archenteron extension depend on Wnt11 family ligands.Developmental biology · 2023Article
- Dishevelled controls bulk cadherin dynamics and the stability of individual cadherin clusters during convergent extension.Molecular biology of the cell · 2022Article
- Microsurgical Methods to Isolate and Culture the Early Gastrula Dorsal Marginal Zone.Cold Spring Harbor protocols · 2022Article
- ARVCF catenin controls force production during vertebrate convergent extension.Developmental cell · 2022Article
- Convergent extension requires adhesion-dependent biomechanical integration of cell crawling and junction contraction.Cell reports · 2022Article
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- Mechanical regulation of early vertebrate embryogenesis.Nature reviews. Molecular cell biology · 2022Review
- Twinfilin1 controls lamellipodial protrusive activity and actin turnover during vertebrate gastrulation.Journal of cell science · 2021Article
- Article
- Cellular processes driving gastrulation in the avian embryo.Mechanisms of development · 2020Review
- From biomechanics to mechanobiology: Xenopus provides direct access to the physical principles that shape the embryo.Current opinion in genetics & development · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Vertebrate embryos undergo dramatic shape changes at gastrulation that require locally produced and anisotropically applied forces, yet how these forces are produced and transmitted across tissues remains unclear. We show that depletion of myosin regulatory light chain (RLC) levels in the embryo blocks force generation at gastrulation through two distinct mechanisms: destabilizing the myosin II (MII) hexameric complex and inhibiting MII contractility. Molecular dissection of these two mechanisms demonstrates that normal convergence force generation requires MII contractility and we identify a set of molecular phenotypes correlated with both this failure of convergence force generation in explants and of blastopore closure in whole embryos. These include reduced rates of actin movement, alterations in C-cadherin dynamics and a reduction in the number of polarized lamellipodia on intercalating cells. By examining the spatial relationship between C-cadherin and actomyosin we also find evidence for formation of transcellular linear arrays incorporating these proteins that could transmit mediolaterally oriented tensional forces. These data combine to suggest a multistep model to explain how cell intercalation can occur against a force gradient to generate axial extension forces. First, polarized lamellipodia extend mediolaterally and make new C-cadherin-based contacts with neighboring mesodermal cell bodies. Second, lamellipodial flow of actin coalesces into a tension-bearing, MII-contractility-dependent node-and-cable actin network in the cell body cortex. And third, this actomyosin network contracts to generate mediolateral convergence forces in the context of these transcellular arrays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.