ArticleJournal of materials science. Materials in medicine2016
Development of three-dimensional tissue engineered bone-oral mucosal composite models.
Article in Journal of materials science. Materials in medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Establishment of a three-dimensional in vitro peri-implant bone-mucosa composite model.BMC oral health · 2026Article
- Article
- Recent trends and perspectives in reconstruction and regeneration of intra/extra-oral wounds using tissue-engineered oral mucosa equivalents.The Japanese dental science review · 2023Review
- Development of fibroblast/endothelial cell-seeded collagen scaffolds for in vitro prevascularization.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- Oral mucosa equivalents, prevascularization approaches, and potential applications.Connective tissue research · 2022Review
- The degradation of gelatin/alginate/fibrin hydrogels is cell type dependent and can be modulated by targeting fibrinolysis.Frontiers in bioengineering and biotechnology · 2022Article
- Vascularization strategies in tissue engineering approaches for soft tissue repair.Journal of tissue engineering and regenerative medicine · 2021Review
- Osteo-mucosal engineered construct: In situ adhesion of hard-soft tissues.Materials science & engineering. C, Materials for biological applications · 2021Article
- A Scarless Healing Tale: Comparing Homeostasis and Wound Healing of Oral Mucosa With Skin and Oesophagus.Frontiers in cell and developmental biology · 2021Review
- Implant Soft-Tissue Attachment Using 3D Oral Mucosal Models-A Pilot Study.Dentistry journal · 2020Article
- Biomodification of a Class-V Restorative Material by Incorporation of Bioactive Agents.Dentistry journal · 2019Article
- Evaluation of a novel oral mucosa in vitro implantation model for analysis of molecular interactions with dental abutment surfaces.Clinical implant dentistry and related research · 2019Article
- Micro-CT - a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results.Biomaterials research · 2018Review
- Cell culture models of oral mucosal barriers: A review with a focus on applications, culture conditions and barrier properties.Tissue barriers · 2018Review
- 3D Printing of Tissue Engineered Constructs for In Vitro Modeling of Disease Progression and Drug Screening.Annals of biomedical engineering · 2017Review
- Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model.Cytotechnology · 2016Article
- In vitro and ex vivo models of the oral mucosa as platforms for the validation of novel drug delivery systems.Journal of tissue engineeringReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue engineering of bone and oral mucosa have been extensively studied independently. The aim of this study was to develop and investigate a novel combination of bone and oral mucosa in a single 3D in vitro composite tissue mimicking the natural structure of alveolar bone with an overlying oral mucosa. Rat osteosarcoma (ROS) cells were seeded into a hydroxyapatite/tri-calcium phosphate scaffold and bone constructs were cultured in a spinner bioreactor for 3 months. An engineered oral mucosa was fabricated by air/liquid interface culture of immortalized OKF6/TERET-2 oral keratinocytes on collagen gel-embedded fibroblasts. EOM was incorporated into the engineered bone using a tissue adhesive and further cultured prior to qualitative and quantitative assessments. Presto Blue assay revealed that ROS cells remained vital throughout the experiment. The histological and scanning electron microscope examinations showed that the cells proliferated and densely populated the scaffold construct. Micro computed tomography (micro-CT) scanning revealed an increase in closed porosity and a decrease in open and total porosity at the end of the culture period. Histological examination of bone-oral mucosa model showed a relatively differentiated parakeratinized epithelium, evenly distributed fibroblasts in the connective tissue layer and widely spread ROS cells within the bone scaffold. The feasibility of fabricating a novel bone-oral mucosa model using cell lines is demonstrated. Generating human 'normal' cell-based models with further characterization is required to optimize the model for in vitro and in vivo applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.