ArticleScientific reports2016
ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 66 citations in OpenAlex.
- In vitro and in vivo assessment of a non-animal sourced chitosan scaffold loaded with xeno-free umbilical cord mesenchymal stromal cells cultured under macromolecular crowding conditions.Biomaterials and biosystems · 2024Article
- Gums as Macromolecular Crowding Agents in Human Skin Fibroblast Cultures.Life (Basel, Switzerland) · 2024Article
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- ATF2-driven osteogenic activity of enoxaparin sodium-loaded polymethylmethacrylate bone cement in femoral defect regeneration.Journal of orthopaedic surgery and research · 2023Article
- TGFBI remodels adipose metabolism by regulating the Notch-1 signaling pathway.Experimental & molecular medicine · 2023Article
- Macromolecular crowding in animal component-free, xeno-free and foetal bovine serum media for human bone marrow mesenchymal stromal cell expansion and differentiation.Frontiers in bioengineering and biotechnology · 2023Article
- A neurogenic signature involving monoamine Oxidase-A controls human thermogenic adipose tissue development.eLife · 2022Article
- Article
- Scaffold-free cell-based tissue engineering therapies: advances, shortfalls and forecast.NPJ Regenerative medicine · 2021Review
- Macromolecular Crowding as a Tool to Screen Anti-fibrotic Drugs: The Scar-in-a-Jar System Revisited.Frontiers in medicine · 2020Article
- The Story of Nanoparticles in Differentiation of Stem Cells into Neural Cells.Neurochemical research · 2019Review
- CFIm25 and alternative polyadenylation: Conflicting roles in cancer.Cancer letters · 2019Review
- Macromolecular crowding tunes 3D collagen architecture and cell morphogenesis.Biomaterials science · 2019Article
- Nanomaterials modulate stem cell differentiation: biological interaction and underlying mechanisms.Journal of nanobiotechnology · 2017Review
- ColXV promotes adipocyte differentiation via inhibiting DNA methylation and cAMP/PKA pathway in mice.Oncotarget · 2017Article
- Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions.Stem cells international · 2017Article
- Cell source, differentiation, functional stimulation, and potential application of human thermogenic adipocytes in vitro.Journal of physiology and biochemistry · 2016Review
- Making microenvironments: A look into incorporating macromolecular crowding into in vitro experiments, to generate biomimetic microenvironments which are capable of directing cell function for tissue engineering applications.Journal of tissue engineeringArticle
- From niche to organoid: Engineering bone tissues through microenvironmental insights.Journal of tissue engineeringReview
- A preliminary preclinical assessment of macromolecular crowding in tissue engineering.Science progressArticle
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Key to realizing the diagnostic and therapeutic potential of human brown/brite adipocytes is the identification of a renewable, easily accessible and safe tissue source of progenitor cells, and an efficacious in vitro differentiation protocol. We show that macromolecular crowding (MMC) facilitates brown adipocyte differentiation in adult human bone marrow mesenchymal stem cells (bmMSCs), as evidenced by substantially upregulating uncoupling protein 1 (UCP1) and uncoupled respiration. Moreover, MMC also induced 'browning' in bmMSC-derived white adipocytes. Mechanistically, MMC creates a 3D extracellular matrix architecture enshrouding maturing adipocytes in a collagen IV cocoon that is engaged by paxillin-positive focal adhesions also at the apical side of cells, without contact to the stiff support structure. This leads to an enhanced matrix-cell signaling, reflected by increased phosphorylation of ATF2, a key transcription factor in UCP1 regulation. Thus, tuning the dimensionality of the microenvironment in vitro can unlock a strong brown potential dormant in bone marrow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.