ArticleJournal of industrial microbiology & biotechnology2016
Enhanced cellulase production by Trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates.
Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 55 citations in OpenAlex.
- Statical optimization of cellulase enzymes production by Trichoderma harzianum PP400831 using response surface methodology and their application in production of 2G bioethanol.Microbial cell factories · 2026Article
- Optimization and Bioreactor Scale-Up of Cellulase Production inInternational journal of molecular sciences · 2025Article
- Evaluation of Film-Forming Properties of α-1,3-Glucan Obtained from "Chicken of the Woods" Mushroom (Molecules (Basel, Switzerland) · 2025Article
- Influence of Varying Pre-Culture Conditions on the Level of Population Heterogeneity in Batch Cultures with anMicroorganisms · 2023Article
- Fungal Proteases as Emerging Biocatalysts to Meet the Current Challenges and Recent Developments in Biomedical Therapies: An Updated Review.Journal of fungi (Basel, Switzerland) · 2022Review
- Article
- Thermotolerance and Cellulolytic Activity of Fungi Isolated from Soils/Waste Materials in the Industrial Region of Nigeria.Current microbiology · 2021Article
- Purification and Enzymatic Properties of a Difunctional Glycoside Hydrolase fromIndian journal of microbiology · 2020Article
- Optimization of high endoglucanase yields production from polypore fungus,Biology open · 2019Article
- Massive lateral transfer of genes encoding plant cell wall-degrading enzymes to the mycoparasitic fungus Trichoderma from its plant-associated hosts.PLoS genetics · 2018Article
- Carbohydrate-active enzymes in Trichoderma harzianum: a bioinformatic analysis bioprospecting for key enzymes for the biofuels industry.BMC genomics · 2017Article
- Potential application of glycerol in the production of plant beneficial microorganisms.Journal of industrial microbiology & biotechnology · 2017Review
- Biorefinery for Glycerol Rich Biodiesel Industry Waste.Indian journal of microbiology · 2016Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of glycerol obtained as an intermediate of the biodiesel manufacturing process as carbon source for microbial growth is a potential alternative strategy for the production of enzymes and other high-value bioproducts. This work evaluates the production of cellulase enzymes using glycerol for high cell density growth of Trichoderma harzianum followed by induction with a cellulosic material. Firstly, the influence of the carbon source used in the pre-culture step was investigated in terms of total protein secretion and fungal morphology. Enzymatic productivity was then determined for cultivation strategies using different types and concentrations of carbon source, as well as different feeding procedures (batch and fed-batch). The best strategy for cellulase production was then further studied on a larger scale using a stirred tank bioreactor. The proposed strategy for cellulase production, using glycerol to achieve high cell density growth followed by induction with pretreated sugarcane bagasse, achieved enzymatic activities up to 2.27 ± 0.37 FPU/mL, 106.40 ± 8.87 IU/mL, and 9.04 ± 0.39 IU/mL of cellulase, xylanase, and β-glucosidase, respectively. These values were 2 times higher when compared to the control experiments using glucose instead of glycerol. This novel strategy proved to be a promising approach for improving cellulolytic enzymes production, and could potentially contribute to adding value to biomass within the biofuels sector.
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