ArticleScientific reports2016
Tissue-based multiphoton analysis of actomyosin and structural responses in human trabecular meshwork.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- Biomechanical properties of the trabecular meshwork in intraocular pressure regulation: a review.International ophthalmology · 2025Review
- Two-photon excitation fluorescence in ophthalmology: safety and improved imaging for functional diagnostics.Frontiers in medicine · 2023Review
- Advancements in high-resolution imaging of the iridocorneal angle.Frontiers in ophthalmology · 2023Review
- Fundamental Biomaterial Considerations in the Development of a 3D Model Representative of Primary Open Angle Glaucoma.Bioengineering (Basel, Switzerland) · 2021Review
- Aqueous outflow regulation - 21st century concepts.Progress in retinal and eye research · 2021Review
- Effects of Netarsudil on Actin-Driven Cellular Functions in Normal and Glaucomatous Trabecular Meshwork Cells: A Live Imaging Study.Journal of clinical medicine · 2020Article
- Phenotypic and Functional Alterations in Tunneling Nanotubes Formed by Glaucomatous Trabecular Meshwork Cells.Investigative ophthalmology & visual science · 2019Article
- High-Resolution, Adaptive Optics Imaging of the Human Trabecular Meshwork In Vivo.Translational vision science & technology · 2019Article
- In vivo two-photon microscopy of the human eye.Scientific reports · 2019Article
- Deep tissue analysis of distal aqueous drainage structures and contractile features.Scientific reports · 2017Article
- [Histological structure of the trabecular meshwork in the eyeball: challenging the traditional concept and preliminary findings in rabbits, rats and mice].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2017Article
- Tunneling Nanotubes are Novel Cellular Structures That Communicate Signals Between Trabecular Meshwork Cells.Investigative ophthalmology & visual science · 2017Article
- Automated circumferential construction of first-order aqueous humor outflow pathways using spectral-domain optical coherence tomography.Journal of biomedical optics · 2017Article
- Toward in vivo two-photon analysis of mouse aqueous outflow structure and function.Experimental eye research · 2017Review
- Aqueous outflow regulation: Optical coherence tomography implicates pressure-dependent tissue motion.Experimental eye research · 2017Review
- Aqueous outflow - A continuum from trabecular meshwork to episcleral veins.Progress in retinal and eye research · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The contractile trabecular meshwork (TM) modulates aqueous humor outflow resistance and intraocular pressure. The primary goal was to visualize and quantify human TM contractile state by analyzing actin polymerization (F-actin) by 2-photon excitation fluorescence imaging (TPEF) in situ. A secondary goal was to ascertain if structural extracellular matrix (ECM) configuration changed with contractility. Viable ex vivo human TM was incubated with latrunculin-A (Lat-A) or vehicle prior to Alexa-568-phalloidin labeling and TPEF. Quantitative image analysis was applied to 2-dimensional (2D) optical sections and 3D image reconstructions. After Lat-A exposure, (a) the F-actin network reorganized as aggregates; (b) F-actin-associated fluorescence intensity was reduced by 48.6% (mean; p = 0.007; n = 8); (c) F-actin 3D distribution was reduced by 68.9% (p = 0.040); (d) ECM pore cross-sectional area and volume were larger by 36% (p = 0.032) and 65% (p = 0.059) respectively and pores appeared more interconnected; (e) expression of type I collagen and elastin, key TM structural ECM proteins, were unaltered (p = 0.54); and (f) tissue viability was unchanged (p = 0.39) relative to vehicle controls. Thus Lat-A-induced reduction of actomyosin contractility was associated with TM porous expansion without evidence of reduced structural ECM protein expression or cellular viability. These important subcellular-level dynamics could be visualized and quantified within human tissue by TPEF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.