ArticleScientific reports2016
Engineering long shelf life multi-layer biologically active surfaces on microfluidic devices for point of care applications.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Reducing hepatitis C diagnostic disparities with a fully automated deep learning-enabled microfluidic system for HCV antigen detection.Science advances · 2025Article
- Review
- Aryl-diazonium salts offer a rapid and cost-efficient method to functionalize plastic microfluidic devices for increased immunoaffinity capture.Advanced materials technologies · 2023Article
- Microscopic Imaging Methods for Organ-on-a-Chip Platforms.Micromachines · 2022Review
- A dual-flow RootChip enables quantification of bi-directional calcium signaling in primary roots.Frontiers in plant science · 2022Article
- Development of a Microfluidic Device for CD4Biosensors · 2021Article
- On-site detection of food and waterborne bacteria - current technologies, challenges, and future directions.Trends in food science & technology · 2021Article
- Exosomal biomarkers for cancer diagnosis and patient monitoring.Expert review of molecular diagnostics · 2020Review
- Development of a multiplex fully automated assay for rapid quantification of CD4Biosensors & bioelectronics · 2019Article
- Microfluidic Chip for Detection of Fungal Infections.ACS omega · 2019Article
- Development of a Flow-Free Magnetic Actuation Platform for an Automated Microfluidic ELISA.RSC advances · 2019Article
- Advances in Diagnostic Methods for Zika Virus Infection.Journal of medical devices · 2018Review
- Molecular Fingerprints of Hemoglobin on a Nanofilm Chip.Sensors (Basel, Switzerland) · 2018Article
- Emerging technologies for home-based semen analysis.Andrology · 2018Review
- Strategies in Ebola virus disease (EVD) diagnostics at the point of care.Critical reviews in microbiology · 2017Review
- Monitoring Neutropenia for Cancer Patients at the Point of Care.Small methods · 2017Article
- Rapid, label-free CD4 testing using a smartphone compatible device.Lab on a chip · 2017Article
- Advances in Candida detection platforms for clinical and point-of-care applications.Critical reviews in biotechnology · 2017Review
- Near future of tumor immunology: Anticipating resistance mechanisms to immunotherapies, a big challenge for clinical trials.Human vaccines & immunotherapeutics · 2017Article
- Paper-based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms.Expert review of molecular diagnostics · 2017Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Although materials and engineered surfaces are broadly utilized in creating assays and devices with wide applications in diagnostics, preservation of these immuno-functionalized surfaces on microfluidic devices remains a significant challenge to create reliable repeatable assays that would facilitate patient care in resource-constrained settings at the point-of-care (POC), where reliable electricity and refrigeration are lacking. To address this challenge, we present an innovative approach to stabilize surfaces on-chip with multiple layers of immunochemistry. The functionality of microfluidic devices using the presented method is evaluated at room temperature for up to 6-month shelf life. We integrated the preserved microfluidic devices with a lensless complementary metal oxide semiconductor (CMOS) imaging platform to count CD4(+) T cells from a drop of unprocessed whole blood targeting applications at the POC such as HIV management and monitoring. The developed immunochemistry stabilization method can potentially be applied broadly to other diagnostic immuno-assays such as viral load measurements, chemotherapy monitoring, and biomarker detection for cancer patients at the POC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.