ArticleScientific reports2016
Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Mild hydrostatic pressure preconditioning improves post-thaw motility of cryopreserved stallion semen.Frontiers in veterinary science · 2026Article
- Quality of fish eggs and production of androgenetic and gynogenetic doubled haploids (DHs).Fish physiology and biochemistry · 2024Review
- Cellular transfection using rapid decrease in hydrostatic pressure.Scientific reports · 2024Article
- Molecular and cellular programs underlying the development of bovine pre-implantation embryos.Reproduction, fertility, and development · 2023Article
- Cryopreservation of Gametes and Embryos and Their Molecular Changes.International journal of molecular sciences · 2021Review
- Pressure-induced changes on the morphology and gene expression in mammalian cells.Biology open · 2021Article
- Production of hypoallergenic milk from DNA-free beta-lactoglobulin (BLG) gene knockout cow using zinc-finger nucleases mRNA.Scientific reports · 2018Article
- Transcriptome Analysis of Rainbow Trout (International journal of genomics · 2018Article
- Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows.Scientific reports · 2017Article
- Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High hydrostatic pressure (HHP) has been used to pre-condition embryos before essential, yet potentially detrimental procedures such as cryopreservation. However, the mechanisms for HHP are poorly understood. We treated bovine blastocysts with three different HHP (40, 60 and 80 MPa) in combination with three recovery periods (0, 1 h, 2 h post HHP). Re-expansion rates were significantly higher at 40 and 60 but lower at 80 MPa after vitrification-warming in the treated groups than controls. Microarray analysis revealed 399 differentially expressed transcripts, representing 254 unique genes, among different groups. Gene ontology analysis indicated that HHP at 40 and 60 MPa promoted embryo competence through down-regulation of genes in cell death and apoptosis, and up-regulation of genes in RNA processing, cellular growth and proliferation. In contrast, 80 MPa up-regulated genes in apoptosis, and down-regulated protein folding and cell cycle-related genes. Moreover, gene expression was also influenced by the length of the recovery time after HHP. The significantly over-represented categories were apoptosis and cell death in the 1 h group, and protein folding, response to unfolded protein and cell cycle in the 2 h group compared to 0 h. Taken together, HHP promotes competence of vitrified bovine blastocysts through modest transcriptional changes.
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