ArticlePlant cell reports2016
Assessment of ptxD gene as an alternative selectable marker for Agrobacterium-mediated maize transformation.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- The functional mechanisms of phosphite and its applications in crop plants.Frontiers in plant science · 2025Review
- Prospects of genetics and breeding for low-phosphate tolerance: an integrated approach from soil to cell.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Review
- Improved the Activity of Phosphite Dehydrogenase and its Application in Plant Biotechnology.Frontiers in bioengineering and biotechnology · 2021Article
- Article
- A novel dominant selection system for plant transgenics based on phosphite metabolism catalyzed by bacterial alkaline phosphatase.PloS one · 2021Article
- The phosphite oxidoreductase gene, ptxD as a bio-contained chloroplast marker and crop-protection tool for algal biotechnology using Chlamydomonas.Applied microbiology and biotechnology · 2020Article
- Insights Into Genetic and Molecular Elements for Transgenic Crop Development.Frontiers in plant science · 2020Article
- Metabolic engineering of phosphite metabolism inBiotechnology for biofuels · 2020Article
- Use of the ptxD gene as a portable selectable marker for chloroplast transformation in Chlamydomonas reinhardtii.Molecular biotechnology · 2019Article
- Co-culturing on dry filter paper significantly increased the efficiency ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Selective fertilization with phosphite allows unhindered growth of cotton plants expressing theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Tailoring crop nutrition to fight weeds.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Developing a flexible, high-efficiency Agrobacterium-mediated sorghum transformation system with broad application.Plant biotechnology journal · 2018Article
- Growth rate rather than growth duration drives growth heterosis in maize B104 hybrids.Plant, cell & environment · 2018Article
- Phosphite: a novel P fertilizer for weed management and pathogen control.Plant biotechnology journal · 2017Review
- ptxD gene in combination with phosphite serves as a highly effective selection system to generate transgenic cotton (Gossypium hirsutum L.).Plant molecular biology · 2017Article
- Risk associated with off-target plant genome editing and methods for its limitation.Emerging topics in life sciences · 2017Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
Abstract
key messageBacterial phosphite oxidoreductase gene and chemical phosphite can be used as a selection system for Agrobacterium -mediated maize transformation. Application of phosphite (Phi) on plants can interfere the plant metabolic system leading to stunted growth and lethality. On the other hand, ectopic expression of the ptxD gene in tobacco and Arabidopsis allowed plants to grow in media with Phi as the sole phosphorous source. The phosphite oxidoreductase (PTXD) enzyme catalyzes the conversion of Phi into phosphate (Pi) that can then be metabolized by plants and utilized as their essential phosphorous source. Here we assess an alternative selectable marker based on a bacterial ptxD gene for Agrobacterium-mediated maize transformation. We compared the transformation frequencies of maize using either the ptxD/Phi selection system or a standard herbicide bar/bialaphos selection system. Two maize genotypes, a transformation amenable hybrid Hi II and an inbred B104, were tested. Transgene presence, insertion copy numbers, and ptxD transcript levels were analyzed and compared. This work demonstrates that the ptxD/Phi selection system can be used for Agrobacterium-mediated maize transformation of both type I and type II callus culture and achieve a comparable frequency as that of the herbicide bar/bialaphos selection system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.