Evidence map›Paper›PMID 26883140›Full record

ArticleFolia microbiologica2016

Phenotypic and genotypic approach to characterize Arcanobacterium pluranimalium isolated from bovine milk samples.

Jörn-Peter Wickhorst, Abdulwahed Ahmed Hassan, Osama Sammra, Reglindis Huber-Schlenstedt, Christoph Lämmler, Ellen Prenger-Berninghoff, Markus Timke, Amir Abdulmawjood

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Article in Folia microbiologica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Complete Genome Sequence ofMicrobiology resource announcements · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jörn-Peter WickhorstInstitut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Schubertstr. 81, 35392, Giessen, Germany.
Abdulwahed Ahmed HassanDepartment of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
Osama SammraInstitut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Schubertstr. 81, 35392, Giessen, Germany.
Reglindis Huber-SchlenstedtTiergesundheitsdienst Bayern e.V. Eutergesundheitsdienst, Senator-Gerauer-Straße 23, 85586, Grub, Germany.
Christoph LämmlerInstitut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Schubertstr. 81, 35392, Giessen, Germany. christoph.laemmler@vetmed.uni-giessen.de.
Ellen Prenger-BerninghoffInstitut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, 35392, Giessen, Germany.
Markus TimkeEntwicklung Bioanalyse, Bruker Daltonik GmbH, Fahrenheitstraße 4, 28359, Bremen, Germany.
Amir AbdulmawjoodInstitut für Lebensmittelqualität und-sicherheit, Stiftung Tierärztliche Hochschule Hannover, 30173, Hannover, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis and genotypically by sequencing the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region (ISR), the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf, and the pluranimaliumlysin encoding gene pla. The latter could also be identified by a loop-mediated isothermal amplification (LAMP) assay. The presented phenotypic and genotypic approaches might support the identification of A. pluranimalium in future and might help to understand the role this species plays in bovine mastitis.

Indexed as

Actinomycetales InfectionsAnimalsArcanobacteriumBacterial ProteinsBacterial Typing TechniquesCattleCluster AnalysisDNA, BacterialDNA, Ribosomal SpacerMastitis, BovineMilkMolecular Diagnostic TechniquesPhylogenyRNA, Ribosomal, 16SSequence Analysis, DNASpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationBacterial ProteinsDNA, BacterialDNA, Ribosomal SpacerRNA, Ribosomal, 16S

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.