ArticleCurrent microbiology2016
High-Efficiency, Two-Step Scarless-Markerless Genome Genetic Modification in Salmonella enterica.
Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Identification of a Novel Regulatory Gene,Microorganisms · 2025Article
- Efficient markerless genetic manipulation ofApplied and environmental microbiology · 2024Article
- Murine Models of Salmonella Infection.Current protocols · 2023Article
- The Loss ofMicroorganisms · 2022Article
- Small noncoding RNA sRNA0426 is involved in regulating biofilm formation in Streptococcus mutans.MicrobiologyOpen · 2020Article
- Development of a Method for Simultaneous Generation of Multiple Genetic Modification inFrontiers in genetics · 2020Article
- Controversy Surrounding the Function of SpiC Protein inFrontiers in microbiology · 2019Review
- Phage Therapy: What Have We Learned?Viruses · 2018Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a two-step method for scarless-markerless genome genetic modification in Salmonella enterica based on the improved suicide plasmid pGMB152. The whole LacZYA gene can provide a lacZ-based blue/white screening strategy for fast selection of double-crossover mutants by allelic exchange. The high efficiency of this genetic engineering strategy permits the study of gene function by gene knockin, site-directed mutagenesis, and gene knockout to construct live attenuated vaccines.
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