ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2016
Genetic mapping and molecular marker development for Pi65(t), a novel broad-spectrum resistance gene to rice blast using next-generation sequencing.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 136 citations in OpenAlex.
- Combining blast resistance and high yield in FScientific reports · 2026Article
- Genetic Interaction Between Bph50 and Bph51 Confers Resistance to Brown Planthopper in Rice.Rice (New York, N.Y.) · 2026Article
- Protein kinase Pi65 regulates rice blast resistance through phosphorylation-dependent signaling and metabolic reprogramming.Frontiers in genetics · 2025Article
- Identification of significant SNPs and candidate loci for blast disease resistance via GWAS and population structure analysis in ARC panel ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Identification of Genomic Regions Associated with Differences in Flowering Time and Inflorescence Architecture betweenInternational journal of molecular sciences · 2024Article
- Allelic variation in rice blast resistance: a pathway to sustainable disease management.Molecular biology reports · 2024Review
- Marker-assisted breeding accelerates the development of multiple-stress-tolerant rice genotypes adapted to wider environments.Frontiers in plant science · 2024Article
- Phenotypic and Genotypic screening of fifty-two rice (Oryza sativa L.) genotypes for desirable cultivars against blast disease.PloS one · 2023Article
- Advancement in the Breeding, Biotechnological and Genomic Tools towards Development of Durable Genetic Resistance against the Rice Blast Disease.Plants (Basel, Switzerland) · 2022Review
- Cloning and functional analysis of the novel rice blast resistance gene Pi65 in japonica rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Recent Progress in Rice Broad-Spectrum Disease Resistance.International journal of molecular sciences · 2021Review
- Understanding Rice-Genes · 2021Article
- The Sorghum (Frontiers in plant science · 2021Article
- Genome wide association studies for japonica rice resistance to blast in field and controlled conditions.Rice (New York, N.Y.) · 2020Article
- Identification of Blast Resistance QTLs Based on Two Advanced Backcross Populations in Rice.Rice (New York, N.Y.) · 2020Article
- Marker Assisted Forward Breeding to Combine Multiple Biotic-Abiotic Stress Resistance/Tolerance in Rice.Rice (New York, N.Y.) · 2020Article
- Bulked Segregant Analysis Coupled with Whole-Genome Sequencing (BSA-Seq) Mapping Identifies a NovelInternational journal of molecular sciences · 2020Article
- Identification and Fine Mapping ofFrontiers in plant science · 2020Article
- Development of KASP Markers and Identification of a QTL Underlying Powdery Mildew Resistance in Melon (Frontiers in plant science · 2020Article
- Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice.Frontiers in plant science · 2020Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageA novel R gene was mapped to a locus on chromosome 11 from 30.42 to 30.85 Mb, which was proven to be efficient in the improvement of rice blast resistance. Rice blast is a devastating fungal disease worldwide. The use of blast resistance (R) genes is the most important approach to control the disease in rice breeding. In the present study, we finely mapped a novel resistance gene Pi65(t), conferring a broad-spectrum resistance to the fungus Magnaporthe oryzae, using bulked segregant analysis in combination with next-generation sequencing technology. Segregation in a doubled haploid (DH) population and a BC1F2 population suggested that resistance to blast in Gangyu129 was likely conferred by a single dominant gene, designated Pi65(t); it was located on chromosome 11 from 30.20 to 31.20 Mb using next-generation sequencing. After screening recombinants with newly developed molecular markers, the region was narrowed down to 0.43 Mb, flanked by SNP-2 and SNP-8 at the physical location from 30.42 to 30.85 Mb based on the Nipponbare reference database in build 5. Using the software QTL IciMapping, Pi65(t) was further mapped to a locus between InDel-1 and SNP-4 with genetic distances of 0.11 and 0.98 cM, respectively. Within this region, 4 predicted R genes were found with nucleotide binding site and leucine-rich repeat (NBS-LRR) domains. We developed molecular markers to genotype 305 DH lines and found that InDel-1 was closely linked with Pi65(t). Using InDel-1, a new rice variety Chuangxin1 containing Pi65(t) was developed, and it is highly resistant to rice blast and produces a high yield in Liaoning province of China. This indicated that Pi65(t) could play a key role in the improvement of rice blast resistance.
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