ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2016
Targeted genotyping-by-sequencing permits cost-effective identification and discrimination of pasture grass species and cultivars.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Phylogenomics of Western Eurasian Tilia: merging GBS datasets to place the Hyrcanian forest limes.BMC plant biology · 2025Article
- Predicting Perennial Ryegrass Cultivars and the Presence of anSensors (Basel, Switzerland) · 2025Article
- Estimation of ryegrass (Frontiers in plant science · 2025Article
- Machine learning solutions for integrating partially overlapping genetic datasets and modelling host-endophyte effects in ryegrass (Frontiers in plant science · 2025Article
- Single Seed Near-Infrared Hyperspectral Imaging for Classification of Perennial Ryegrass Seed.Sensors (Basel, Switzerland) · 2023Article
- RAD-seq as an effective strategy for heterogenous variety identification in plants-a case study in Italian Ryegrass (Lolium multiflorum).BMC plant biology · 2022Article
- Genomic prediction in family bulks using different traits and cross-validations in pine.G3 (Bethesda, Md.) · 2021Article
- An Assessment of theMicroorganisms · 2021Article
- Genomic interventions for sustainable agriculture.Plant biotechnology journal · 2020Review
- Article
- Integrating High-Throughput Phenotyping and Statistical Genomic Methods to Genetically Improve Longitudinal Traits in Crops.Frontiers in plant science · 2020Review
- Targeted genotyping by sequencing: a new way to genome profile the cat.Animal genetics · 2019Article
- Does the "high sugar" trait of perennial ryegrass cultivars express under temperate climate conditions?Grass and forage science : the journal of the British Grassland Society · 2019Article
- Article
- Genetic Mapping and Prediction Analysis of FHB Resistance in a Hard Red Spring Wheat Breeding Population.Frontiers in plant science · 2019Article
- Exploitation of data from breeding programs supports rapid implementation of genomic selection for key agronomic traits in perennial ryegrass.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Toward Genomics-Based Breeding in C3 Cool-Season Perennial Grasses.Frontiers in plant science · 2017Review
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
key messageA targeted amplicon-based genotyping-by-sequencing approach has permitted cost-effective and accurate discrimination between ryegrass species (perennial, Italian and inter-species hybrid), and identification of cultivars based on bulked samples. Perennial ryegrass and Italian ryegrass are the most important temperate forage species for global agriculture, and are represented in the commercial pasture seed market by numerous cultivars each composed of multiple highly heterozygous individuals. Previous studies have identified difficulties in the use of morphophysiological criteria to discriminate between these two closely related taxa. Recently, a highly multiplexed single nucleotide polymorphism (SNP)-based genotyping assay has been developed that permits accurate differentiation between both species and cultivars of ryegrasses at the genetic level. This assay has since been further developed into an amplicon-based genotyping-by-sequencing (GBS) approach implemented on a second-generation sequencing platform, allowing accelerated throughput and ca. sixfold reduction in cost. Using the GBS approach, 63 cultivars of perennial, Italian and interspecific hybrid ryegrasses, as well as intergeneric Festulolium hybrids, were genotyped. The genetic relationships between cultivars were interpreted in terms of known breeding histories and indistinct species boundaries within the Lolium genus, as well as suitability of current cultivar registration methodologies. An example of applicability to quality assurance and control (QA/QC) of seed purity is also described. Rapid, low-cost genotypic assays provide new opportunities for breeders to more fully explore genetic diversity within breeding programs, allowing the combination of novel unique genetic backgrounds. Such tools also offer the potential to more accurately define cultivar identities, allowing protection of varieties in the commercial market and supporting processes of cultivar accreditation and quality assurance.
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