ArticleGenome biology2016
Variation in 5-hydroxymethylcytosine across human cortex and cerebellum.
Article in Genome biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 57 papers, 2 of them syntheses that pooled it.
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Who cites it
57 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- A Meta-Analysis of Brain DNA Methylation Across Sex, Age, and Alzheimer's Disease Points for Accelerated Epigenetic Aging in Neurodegeneration.Frontiers in aging neuroscience · 2021Pooled it
- Genome-wide DNA methylation meta-analysis in the brains of suicide completers.Translational psychiatry · 2020Pooled it
- Loss of epigenetic adaptation to a high-fat diet in alpha-synuclein transgenic mice.Frontiers in cellular neuroscience · 2026Article
- Epigenetic landscape of 5-hydroxymethylcytosine and associations with gene expression in placenta.Epigenetics · 2024Article
- Genome-wide methylomic regulation of multiscale gene networks in Alzheimer's disease.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2023Article
- Epigenetics in psychiatry: Beyond DNA methylation.World journal of psychiatry · 2023Review
- Altered Brain Expression of DNA Methylation and Hydroxymethylation Epigenetic Enzymes in a Rat Model of Neuropathic Pain.International journal of molecular sciences · 2023Article
- A pilot investigation of differential hydroxymethylation levels in patient-derived neural stem cells implicates altered cortical development in bipolar disorder.Frontiers in psychiatry · 2023Article
- Alpha-synuclein overexpression induces epigenomic dysregulation of glutamate signaling and locomotor pathways.Human molecular genetics · 2022Article
- Evaluation of nanopore sequencing for epigenetic epidemiology: a comparison with DNA methylation microarrays.Human molecular genetics · 2022Article
- The Epigenetics of Psychosis: A Structured Review with Representative Loci.Biomedicines · 2022Review
- DNA methylation as a mediator of genetic and environmental influences on Parkinson's disease susceptibility: Impacts of alpha-Synuclein, physical activity, and pesticide exposure on the epigenome.Frontiers in genetics · 2022Review
- Neurodegenerative movement disorders: An epigenetics perspective and promise for the future.Neuropathology and applied neurobiology · 2021Review
- Epigenetic age prediction.Aging cell · 2021 · on this mapReview
- Bisulfite Amplicon Sequencing Can Detect Glia and Neuron Cell-Free DNA in Blood Plasma.Frontiers in molecular neuroscience · 2021Article
- Genome-Wide DNA Methylation and Hydroxymethylation Changes Revealed Epigenetic Regulation of Neuromodulation and Myelination in Yak Hypothalamus.Frontiers in genetics · 2021Article
- The Impact of Environmental Factors on 5-Hydroxymethylcytosine in the Brain.Current environmental health reports · 2020Review
- Genome-wide characterization of cytosine-specific 5-hydroxymethylation in normal breast tissue.Epigenetics · 2020Article
- Epigenetic modulation of AREL1 and increased HLA expression in brains of multiple system atrophy patients.Acta neuropathologica communications · 2020Article
- Systematic underestimation of the epigenetic clock and age acceleration in older subjects.Genome biology · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
Abstract
backgroundThe most widely utilized approaches for quantifying DNA methylation involve the treatment of genomic DNA with sodium bisulfite; however, this method cannot distinguish between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Previous studies have shown that 5hmC is enriched in the brain, although little is known about its genomic distribution and how it differs between anatomical regions and individuals. In this study, we combine oxidative bisulfite (oxBS) treatment with the Illumina Infinium 450K BeadArray to quantify genome-wide patterns of 5hmC in two distinct anatomical regions of the brain from multiple individuals.
resultsWe identify 37,145 and 65,563 sites passing our threshold for detectable 5hmC in the prefrontal cortex and cerebellum respectively, with 23,445 loci common across both brain regions. Distinct patterns of 5hmC are identified in each brain region, with notable differences in the genomic location of the most hydroxymethylated loci between these brain regions. Tissue-specific patterns of 5hmC are subsequently confirmed in an independent set of prefrontal cortex and cerebellum samples.
conclusionsThis study represents the first systematic analysis of 5hmC in the human brain, identifying tissue-specific hydroxymethylated positions and genomic regions characterized by inter-individual variation in DNA hydroxymethylation. This study demonstrates the utility of combining oxBS-treatment with the Illumina 450k methylation array to systematically quantify 5hmC across the genome and the potential utility of this approach for epigenomic studies of brain disorders.
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