ArticleScientific reports2016
Long-term safety and stability of angiogenesis induced by balanced single-vector co-expression of PDGF-BB and VEGF164 in skeletal muscle.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 38 citations in OpenAlex.
- Impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of COPD patients.Respiratory research · 2019Trial
- Platelet concentrates integrated with tissue engineering scaffolds: a promising approach for wound healing application.Journal of nanobiotechnology · 2026Review
- Assessing the Angiogenic Potential of Poly(ε-Caprolactone) PCL/Bioactive Glass Composites in a Co-Culture Model of ASCs and HMEC-1.Biomedicines · 2026Article
- Article
- Biomechanical characteristics of frozen bone allografts after gene therapy-induced revascularization.Academia biology · 2025Article
- Engineered myovascular tissues for studies of endothelial/satellite cell interactions.Acta biomaterialia · 2024Article
- Near-infrared light-responsive hydrogels for on-demand dual delivery of proangiogenic growth factors.Acta biomaterialia · 2024Article
- Macrophages upregulate mural cell-like markers and support healing of ischemic injury by adopting functions important for vascular support.Nature cardiovascular research · 2024Article
- Tissue-engineered small-diameter vascular grafts containing novel copper-doped bioactive glass biomaterials to promote angiogenic activity and endothelial regeneration.Materials today. Bio · 2023Article
- Vasculogenic gene therapy: No role for revitalization of structural bone allografts.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Comparison of the Behavior of Perivascular Cells (Pericytes and CD34+ Stromal Cell/Telocytes) in Sprouting and Intussusceptive Angiogenesis.International journal of molecular sciences · 2022Review
- Mechanical Aspects of Angiogenesis.Cancers · 2021Review
- Robust Angiogenesis and Arteriogenesis in the Skin of Diabetic Mice by Transient Delivery of Engineered VEGF and PDGF-BB Proteins in Fibrin Hydrogels.Frontiers in bioengineering and biotechnology · 2021Article
- Effects of platelet-derived growth factor-BB on cellular morphology and cellular viability of stem cell spheroids composed of bone-marrow-derived stem cells.Biomedical reports · 2020Article
- Intussusceptive angiogenesis and its counterpart intussusceptive lymphangiogenesis.Histology and histopathology · 2020Review
- Therapeutic vascularization in regenerative medicine.Stem cells translational medicine · 2020Review
- Balanced single-vector co-delivery of VEGF/PDGF-BB improves functional collateralization in chronic cerebral ischemia.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Article
- Vascular Tumors Result from Adeno-Associated Virus-9 Angiogenic Gene Therapy of Bone Allografts.Vascular cell · 2020Article
- State-of-the-Art Strategies for the Vascularization of Three-Dimensional Engineered Organs.Vascular specialist international · 2019Review
- Therapeutic vascular growth in the heart.Vascular biology (Bristol, England) · 2019Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Therapeutic angiogenesis by growth factor delivery is an attractive treatment strategy for ischemic diseases, yet clinical efficacy has been elusive. The angiogenic master regulator VEGF-A can induce aberrant angiogenesis if expressed above a threshold level. Since VEGF remains localized in the matrix around expressing cells, homogeneous dose distribution in target tissues is required, which is challenging. We found that co-expression of the pericyte-recruiting factor PDGF-BB at a fixed ratio with VEGF from a single bicistronic vector ensured normal angiogenesis despite heterogeneous high VEGF levels. Taking advantage of a highly controlled gene delivery platform, based on monoclonal populations of transduced myoblasts, in which every cell stably produces the same amount of each factor, here we rigorously investigated a) the dose-dependent effects, and b) the long-term safety and stability of VEGF and PDGF-BB co-expression in skeletal muscle. PDGF-BB co-expression did not affect the normal angiogenesis by low and medium VEGF doses, but specifically prevented vascular tumors by high VEGF, yielding instead normal and mature capillary networks, accompanied by robust arteriole formation. Induced angiogenesis persisted unchanged up to 4 months, while no tumors appeared. Therefore, PDGF-BB co-expression is an attractive strategy to improve safety and efficacy of therapeutic angiogenesis by VEGF gene delivery.
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