ArticleOrganic & biomolecular chemistry2016
One-pot multienzyme (OPME) systems for chemoenzymatic synthesis of carbohydrates.
Article in Organic & biomolecular chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 142 citations in OpenAlex.
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- Molecular mechanism of decision-making in glycosaminoglycan biosynthesis.Nature communications · 2023Article
- A concise chemoenzymatic total synthesis of neutral Globo-series glycosphingolipids Globo A and Globo B, and Forssman and para-Forssman antigens.Glycoconjugate journal · 2023Article
- A Hitchhiker's Guide to Problem Selection in Carbohydrate Synthesis.ACS central science · 2023Review
- Glycoprotein In Vitro N-Glycan Processing Using Enzymes Expressed inMolecules (Basel, Switzerland) · 2023Article
- Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis.JACS Au · 2023Review
- Digital microfluidics-engaged automated enzymatic degradation and synthesis of oligosaccharides.Frontiers in bioengineering and biotechnology · 2023Article
- Tools for mammalian glycoscience research.Cell · 2022Review
- Small tools for sweet challenges: advances in microfluidic technologies for glycan synthesis.Analytical and bioanalytical chemistry · 2022Review
- Substrate and Process Engineering for Biocatalytic Synthesis and Facile Purification of Human Milk Oligosaccharides.ChemSusChem · 2022Article
- Deracemization of 1-phenylethanols in a one-pot process combining Mn-driven oxidation with enzymatic reduction utilizing a compartmentalization technique.RSC advances · 2022Article
- Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases.International journal of molecular sciences · 2022Review
- Cell-Free Multi-Enzyme Synthesis and Purification of Uridine Diphosphate Galactose.Chembiochem : a European journal of chemical biology · 2022Article
- Engineering analysis of multienzyme cascade reactions for 3'-sialyllactose synthesis.Biotechnology and bioengineering · 2021Article
- Recent progress in synthesis of carbohydrates with sugar nucleotide-dependent glycosyltransferases.Current opinion in chemical biology · 2021Review
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Authors and funding
2 authors at 2 institutions in 1 country.
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Abstract
Glycosyltransferase-catalyzed enzymatic and chemoenzymatic syntheses are powerful approaches for the production of oligosaccharides, polysaccharides, glycoconjugates, and their derivatives. Enzymes involved in the biosynthesis of sugar nucleotide donors can be combined with glycosyltransferases in one pot for efficient production of the target glycans from simple monosaccharides and acceptors. The identification of enzymes involved in the salvage pathway of sugar nucleotide generation has greatly facilitated the development of simplified and efficient one-pot multienzyme (OPME) systems for synthesizing major glycan epitopes in mammalian glycomes. The applications of OPME methods are steadily gaining popularity mainly due to the increasing availability of wild-type and engineered enzymes. Substrate promiscuity of these enzymes and their mutants allows OPME synthesis of carbohydrates with naturally occurring post-glycosylational modifications (PGMs) and their non-natural derivatives using modified monosaccharides as precursors. The OPME systems can be applied in sequence for synthesizing complex carbohydrates. The sequence of the sequential OPME processes, the glycosyltransferase used, and the substrate specificities of the glycosyltransferases define the structures of the products. The OPME and sequential OPME strategies can be extended to diverse glycans in other glycomes when suitable enzymes with substrate promiscuity become available. This Perspective summarizes the work of the authors and collaborators on the development of glycosyltransferase-based OPME systems for carbohydrate synthesis. Future directions are also discussed.
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