Evidence map›Paper›PMID 26880982›Full record

ArticleStem cells international2016

Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering.

Bing Song, Wenkai Jiang, Amr Alraies, Qian Liu, Vijay Gudla, Julia Oni, Xiaoqing Wei, Alastair Sloan, Longxing Ni, Meena Agarwal

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
5.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 44 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Dental-Derived Mesenchymal Stem Cells: State of the Art.Frontiers in cell and developmental biology · 2021
    Review
  17. Review
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Bing SongSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UK; State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Shaanxi 710032, China.
Wenkai JiangSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UK; State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Shaanxi 710032, China.
Amr AlraiesSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UK.
Qian LiuSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UK.
Vijay GudlaDepartment of Urology/Surgery, Cardiff University and University Hospital of Wales, Heath Park, Cardiff CF14 4XW, UK.
Julia OniDepartment of Urology/Surgery, Cardiff University and University Hospital of Wales, Heath Park, Cardiff CF14 4XW, UK.
Xiaoqing WeiSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UK.
Alastair SloanSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UK; State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Shaanxi 710032, China.
Longxing NiState Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Shaanxi 710032, China.
Meena AgarwalDepartment of Urology/Surgery, Cardiff University and University Hospital of Wales, Heath Park, Cardiff CF14 4XW, UK.
Cardiff University · GBAir Force Medical University · CNUniversity Hospital of Wales · GB

Funding

European Research Council 243261Medical Research Council G0900954
6 · The paper itself

Abstract

Dental pulp stem cells (DPSCs) are multipotent cells capable of differentiating into multiple cell lines, thus providing an alternative source of cell for tissue engineering. Smooth muscle cell (SMC) regeneration is a crucial step in tissue engineering of the urinary bladder. It is known that DPSCs have the potential to differentiate into a smooth muscle phenotype in vitro with differentiation agents. However, most of these studies are focused on the vascular SMCs. The optimal approaches to induce human DPSCs to differentiate into bladder SMCs are still under investigation. We demonstrate in this study the ability of human DPSCs to differentiate into bladder SMCs in a growth environment containing bladder SMCs-conditioned medium with the addition of the transforming growth factor beta 1 (TGF-β1). After 14 days of exposure to this medium, the gene and protein expression of SMC-specific marker (α-SMA, desmin, and calponin) increased over time. In particular, myosin was present in differentiated cells after 11 days of induction, which indicated that the cells differentiated into the mature SMCs. These data suggested that human DPSCs could be used as an alternative and less invasive source of stem cells for smooth muscle regeneration, a technology that has applications for bladder tissue engineering.

Identifiers

PMID26880982
PMCPMC4736571
OpenAlexW2227539792

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.