ArticleThe Journal of general physiology2016
Regulation of ENaC trafficking in rat kidney.
Article in The Journal of general physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 53 citations in OpenAlex.
- ENaC processing in rat kidney: effects of salt loading and ADH.American journal of physiology. Renal physiology · 2026Article
- Processing of ENaC in mouse kidney: effects of aldosterone and a Liddle syndrome mutation.American journal of physiology. Renal physiology · 2026Article
- Kinetics of aldosterone-dependent ENaC trafficking in the kidney.The Journal of general physiology · 2025Article
- Acute activation of human epithelial sodium channel (ENaC) by serum and glucocorticoid inducible kinase 1 (SGK1) requires prior cleavage of the channel's γ-subunit at its proximal cleavage site.Pflugers Archiv : European journal of physiology · 2025Article
- Transmembrane Serine Protease 2 and Proteolytic Activation of the Epithelial Sodium Channel in Mouse Kidney.Journal of the American Society of Nephrology : JASN · 2025Article
- Control of ENaC ubiquitination.American journal of physiology. Renal physiology · 2024Article
- Influence of proteolytic cleavage of ENaC's γ subunit upon NaAmerican journal of physiology. Renal physiology · 2024Article
- Epithelial NaComprehensive Physiology · 2024Review
- High dietary KAmerican journal of physiology. Renal physiology · 2023Article
- Does the composition of urinary extracellular vesicles reflect the abundance of renal NaPflugers Archiv : European journal of physiology · 2022Article
- Expression of ENaC subunits in epithelia.The Journal of general physiology · 2022Article
- Cleavage state of γENaC in mouse and rat kidneys.American journal of physiology. Renal physiology · 2021Article
- Antiproteinuric and Hyperkalemic Mechanisms Activated by Dual Versus Single Blockade of the RAS in Renovascular Hypertensive Rats.Frontiers in physiology · 2021Article
- Plasminogen deficiency does not prevent sodium retention in a genetic mouse model of experimental nephrotic syndrome.Acta physiologica (Oxford, England) · 2021Article
- Aldosterone-dependent and -independent regulation of NaAmerican journal of physiology. Renal physiology · 2020Article
- Ubiquitination of renal ENaC subunits in vivo.American journal of physiology. Renal physiology · 2020Article
- The epithelial NaPflugers Archiv : European journal of physiology · 2019Article
- Trichostatin A blocks aldosterone-induced NaBritish journal of pharmacology · 2019Article
- The epithelial NaThe Journal of biological chemistry · 2018Article
- Regulation of renal Na transporters in response to dietary K.American journal of physiology. Renal physiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The epithelial Na channel (ENaC) forms a pathway for Na(+) reabsorption in the distal nephron, and regulation of these channels is essential for salt homeostasis. In the rat kidney, ENaC subunits reached the plasma membrane in both immature and fully processed forms, the latter defined by either endoglycosidase H-insensitive glycosylation or proteolytic cleavage. Animals adapted to a low-salt diet have increased ENaC surface expression that is specific for the mature forms of the subunit proteins and is similar (three- to fourfold) for α, β, and γENaC. Kidney membranes were fractionated using differential centrifugation, sucrose-gradient separation, and immunoabsorption. Endoplasmic reticulum membranes, isolated using an antibody against calnexin, expressed immature γENaC, and the content decreased with Na depletion. Golgi membranes, isolated with an antibody against the cis-Golgi protein GM130, expressed both immature and processed γENaC; Na depletion increased the content of processed γENaC in this fraction by 3.8-fold. An endosomal compartment isolated using an antibody against Rab11 contained both immature and processed γENaC; the content of processed subunit increased 2.4-fold with Na depletion. Finally, we assessed the content of γENaC in the late endocytic compartments indirectly using urinary exosomes. All of the γENaC in these exosomes was in the fully cleaved form, and its content increased by 4.5-fold with Na depletion. These results imply that stimulation of ENaC surface expression results at least in part from increased rates of formation of fully processed subunits in the Golgi and subsequent trafficking to the apical membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.