Evidence map›Paper›PMID 26880140›Full record

ArticleStem cell reviews and reports2016

Mesenchymal Stem/Stromal Cells Derived From a Reproductive Tissue Niche Under Oxidative Stress Have High Aldehyde Dehydrogenase Activity.

Gina D Kusuma, Mohamed H Abumaree, Mark D Pertile, Anthony V Perkins, Shaun P Brennecke, Bill Kalionis

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In one paragraph

Article in Stem cell reviews and reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
5.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 47 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Gina D KusumaDepartment of Obstetrics and Gynaecology, University of Melbourne, Royal Women's Hospital, Parkville, VIC, 3052, Australia.
Mohamed H AbumareeKing Abdullah International Medical Research Center/ King Saud Bin Abdulaziz University for Health Sciences, College of Science and Health Professions, King Abdulaziz Medical City - National Guard Health Affairs, P.O. Box 3660, Riyadh, 11481, Mail Code 3124, Kingdom of Saudi Arabia.
Mark D PertileVictorian Clinical Genetics Services, Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road, Parkville, VIC, 3052, Australia.
Anthony V PerkinsSchool of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, QLD, 9726, Australia.
Shaun P BrenneckeDepartment of Obstetrics and Gynaecology, University of Melbourne, Royal Women's Hospital, Parkville, VIC, 3052, Australia.
Bill KalionisDepartment of Obstetrics and Gynaecology, University of Melbourne, Royal Women's Hospital, Parkville, VIC, 3052, Australia. Bill.kalionis@thewomens.org.au.
Royal Women's Hospital · AUGriffith University · AUKing Abdulaziz Medical City · SARoyal Children's Hospital · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The use of mesenchymal stem/stromal cells (MSC) in regenerative medicine often requires MSC to function in environments of high oxidative stress. Human pregnancy is a condition where the mother's tissues, and in particular her circulatory system, are exposed to increased levels of oxidative stress. MSC in the maternal decidua basalis (DMSC) are in a vascular niche, and thus would be exposed to oxidative stress products in the maternal circulation. Aldehyde dehydrogenases (ALDH) are a large family of enzymes which detoxify aldehydes and thereby protect stem cells against oxidative damage. A subpopulation of MSC express high levels of ALDH (ALDH(br)) and these are more potent in repairing and regenerating tissues. DMSC was compared with chorionic villous MSC (CMSC) derived from the human placenta. CMSC reside in vascular niche and are exposed to the fetal circulation, which is in lower oxidative state. We screened an ALDH isozyme cDNA array and determined that relative to CMSC, DMSC expressed high levels of ALDH1 family members, predominantly ALDH1A1. Immunocytochemistry gave qualitative confirmation at the protein level. Immunofluorescence detected ALDH1 immunoreactivity in the DMSC and CMSC vascular niche. The percentage of ALDH(br) cells was calculated by Aldefluor assay and DMSC showed a significantly higher percentage of ALDH(br) cells than CMSC. Finally, flow sorted ALDH(br) cells were functionally potent in colony forming unit assays. DMSC, which are derived from pregnancy tissues that are naturally exposed to high levels of oxidative stress, may be better candidates for regenerative therapies where MSC must function in high oxidative stress environments.

Indexed as

Aldehyde DehydrogenaseCell DifferentiationColony-Forming Units AssayFemaleFlow CytometryHumansMesenchymal Stem CellsOxidative StressPlacentaPregnancyRegenerationStem CellsAldehyde DehydrogenaseAldehyde dehydrogenaseChorionic villiDeciduaMesenchymal stem cellsPlacenta

Identifiers

PMID26880140
OpenAlexW2280938069

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.