ArticleScientific reports2016
Development of non-defective recombinant densovirus vectors for microRNA delivery in the invasive vector mosquito, Aedes albopictus.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Biopesticides as an eco-friendly approach to mosquitoes and their role in reducing transmission of vector-borne disease: updates and prospects.Pest management science · 2026Review
- Article
- Expression of endogenous Anopheles gambiae microRNAs using an Anopheles gambiae densovirus (AgDNV) intronic expression system.Parasites & vectors · 2025Article
- Challenges in developing a split drive targeting dsx for the genetic control of the invasive malaria vector Anopheles stephensi.Parasites & vectors · 2025Article
- Generating prophylactic immunity against arboviruses in vertebrates and invertebrates.Nature reviews. Immunology · 2024Review
- Mosquito densovirus significantly reduces the vector susceptibility to dengue virus serotype 2 in Aedes albopictus mosquitoes (Diptera: Culicidae).Infectious diseases of poverty · 2023Article
- Armigeres subalbatus is a potential vector for Zika virus but not dengue virus.Infectious diseases of poverty · 2022Article
- Intron-derived small RNAs for silencing viral RNAs in mosquito cells.PLoS neglected tropical diseases · 2022Article
- Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors.Parasites & vectors · 2022Review
- Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity againstViruses · 2022Article
- Recombinant Mosquito Densovirus withToxins · 2022Article
- Paratransgenic manipulation of a tsetse microRNA alters the physiological homeostasis of the fly's midgut environment.PLoS pathogens · 2021Article
- Mosquito Trilogy: Microbiota, Immunity and Pathogens, and Their Implications for the Control of Disease Transmission.Frontiers in microbiology · 2021Review
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
- Development of large-scale mosquito densovirus production by in vivo methods.Parasites & vectors · 2019Article
- A Novel Densovirus Isolated From the Asian Tiger Mosquito Displays Varied Pathogenicity Depending on Its Host Species.Frontiers in microbiology · 2019Article
- Densonucleosis viruses ('densoviruses') for mosquito and pathogen control.Current opinion in insect science · 2018Review
- Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae.Journal of visualized experiments : JoVE · 2017Article
- Microbial control of arthropod-borne disease.Memorias do Instituto Oswaldo Cruz · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported that mosquito densoviruses (MDVs) are potential vectors for delivering foreign nucleic acids into mosquito cells. However, considering existing expression strategies, recombinant viruses would inevitably become replication-defective viruses and lose their ability for secondary transmission. The packaging limitations of the virion represent a barrier for the development of MDVs for viral paratransgenesis or as high-efficiency bioinsecticides. Herein, we report the development of a non-defective recombinant Aedes aegypti densovirus (AaeDV) miRNA expression system, mediated by an artificial intron, using an intronic miRNA expression strategy. We demonstrated that this recombinant vector could be used to overexpress endogenous miRNAs or to decrease endogenous miRNAs by generating antisense sponges to explore the biological functions of miRNAs. In addition, the vector could express antisense-miRNAs to induce efficient gene silencing in vivo and in vitro. The recombinant virus effectively self-replicated and retained its secondary transmission ability, similar to the wild-type virus. The recombinant virus was also genetically stable. This study demonstrated the first construction of a non-defective recombinant MDV miRNA expression system, which represents a tool for the functional analysis of mosquito genes and lays the foundation for the application of viral paratransgenesis for dengue virus control.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.