ArticleBMC musculoskeletal disorders2016
Profiling microRNA expression during fracture healing.
Article in BMC musculoskeletal disorders, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.
- Prognostic and therapeutic potential of microRNAs for fracture healing processes and non-union fractures: A systematic review.Clinical and translational medicine · 2023Pooled it
- Review
- Epigenetic Regulation of Bone Healing: Implications for Fracture Repair and Clinical Treatment Strategies.The Yale journal of biology and medicine · 2025Review
- Functional mechanism and clinical implications of mir-1271-5p in pilon fracture healing processes.Journal of orthopaedic surgery and research · 2024Article
- MiR-331-3p facilitates osteoporosis and may promote osteoporotic fractures by modulating NRP2 expression.Journal of orthopaedic surgery and research · 2024Article
- MiR-22-3p facilitates bone marrow mesenchymal stem cell osteogenesis and fracture healing through the SOSTDC1-PI3K/AKT pathway.International journal of experimental pathology · 2024Article
- Microgravity's effects on miRNA-mRNA regulatory networks in a mouse model of segmental bone defects.PloS one · 2024Article
- Identification of miRNAs related to osteoporosis by high-throughput sequencing.Frontiers in pharmacology · 2024Article
- Article
- The miR-181 family: Wide-ranging pathophysiological effects on cell fate and function.Journal of cellular physiology · 2023Review
- Identification of the miRNAome in human fracture callus and nonunion tissues.Journal of orthopaedic translation · 2023Article
- MicroRNA-181a-5p Curbs Osteogenic Differentiation and Bone Formation Partially Through Impairing Runx1-Dependent Inhibition of AIF-1 Transcription.Endocrinology and metabolism (Seoul, Korea) · 2023Article
- Signature of circulating small non-coding RNAs during early fracture healing in mice.Bone reports · 2022Article
- Review
- Increased BMSC exosomal miR-140-3p alleviates bone degradation and promotes bone restoration by targeting Plxnb1 in diabetic rats.Journal of nanobiotechnology · 2022Article
- Differential miRNA Expression in Osteoporotic Elderly Patients with Hip Fractures Compared to Young Patients.Indian journal of orthopaedics · 2022Article
- miR-140-3p enhanced the osteo/odontogenic differentiation of DPSCs via inhibiting KMT5B under hypoxia condition.International journal of oral science · 2021Article
- MiRNA-Nanofiber, the Next Generation of Bioactive Scaffolds for Bone Regeneration: A Review.Micromachines · 2021Review
- A database on differentially expressed microRNAs during rodent bladder healing.Scientific reports · 2021Article
- A CREB1-miR-181a-5p loop regulates the pathophysiologic features of bone marrow stromal cells in fibrous dysplasia of bone.Molecular medicine (Cambridge, Mass.) · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe discovery of microRNA (miRNA) has revealed a novel type of regulatory control for gene expression. Increasing evidence suggests that miRNA regulates chondrocyte, osteoblast, and osteoclast differentiation and function, indicating miRNA as key regulators of bone formation, resorption, remodeling, and repair. We hypothesized that the functions of certain miRNAs and changes to their expression pattern may play crucial roles during the process of fracture healing.
methodsStandard healing fractures and unhealing fractures produced by periosteal cauterization at the fracture site were created in femurs of seventy rats, with half assigned to the standard healing fracture group and half assigned to the nonunion group. At post-fracture days 3, 7, 10, 14, 21, and 28, total RNA including miRNA was extracted from the newly generated tissue at the fracture site. Microarray analysis was performed with miRNA samples from each group on post-fracture day 14. For further analysis, we selected highly up-regulated five miRNAs in the standard healing fracture group from the microarray data. Real-time PCR was performed with miRNA samples at each time point above mentioned to compare the expression levels of the selected miRNAs between standard healing fractures and unhealing fractures and investigate their time-course changes.
resultsMicroarray and real-time polymerase chain reaction (PCR) analyses on day 14 revealed that five miRNAs, miR-140-3p, miR-140-5p, miR-181a-5p, miR-181d-5p, and miR-451a, were significantly highly expressed in standard healing fractures compared with unhealing fractures. Real-time PCR analysis further revealed that in standard healing fractures, the expression of all five of these miRNAs peaked on day 14 and declined thereafter.
conclusionOur results suggest that the five miRNAs identified using microarray and real-time PCR analyses may play important roles during fracture healing. These findings provide valuable information to further understand the molecular mechanism of fracture healing and may lead to the development of miRNA-based tissue engineering strategies to promote fracture healing.
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Registered trials
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