ArticleBMC genomics2016
Uncovering the pathways underlying whole body regeneration in a chordate model, Botrylloides leachi using de novo transcriptome analysis.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Evolution and Function of the Notch Signaling Pathway: An Invertebrate Perspective.International journal of molecular sciences · 2024Review
- De novo genome assembly and comparative genomics for the colonial ascidian Botrylloides violaceus.G3 (Bethesda, Md.) · 2023Article
- Using RNA-Seq for Transcriptome Profiling of Botrylloides sp. Regeneration.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Studying Tunicata WBR Using Botrylloides anceps.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Studying Regeneration in Ascidians: An Historical Overview.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Article
- Phylogenomic and morphological relationships among the botryllid ascidians (Subphylum Tunicata, Class Ascidiacea, Family Styelidae).Scientific reports · 2021Article
- Animal regeneration in the era of transcriptomics.Cellular and molecular life sciences : CMLS · 2021Review
- Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology.Frontiers in immunology · 2021Review
- Integrin-alpha-6+ Candidate stem cells are responsible for whole body regeneration in the invertebrate chordate Botrylloides diegensis.Nature communications · 2020Article
- ANISEED 2019: 4D exploration of genetic data for an extended range of tunicates.Nucleic acids research · 2020Article
- Beyond Adult Stem Cells: Dedifferentiation as a Unifying Mechanism Underlying Regeneration in Invertebrate Deuterostomes.Frontiers in cell and developmental biology · 2020Review
- Convergent Acquisition of Nonembryonic Development in Styelid Ascidians.Molecular biology and evolution · 2018Article
- De novo draft assembly of the Botrylloides leachii genome provides further insight into tunicate evolution.Scientific reports · 2018Article
- Transcriptome Analysis Reveals the Genetic Basis of the Resveratrol Biosynthesis Pathway in an Endophytic Fungus (Alternaria sp. MG1) Isolated from Vitis vinifera.Frontiers in microbiology · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRegenerative capacity differs greatly between animals. In vertebrates regenerative abilities are highly limited and tissue or organ specific. However the closest related chordate to the vertebrate clade, Botrylloides leachi, can undergo whole body regeneration (WBR). Therefore, research on WBR in B. leachi has focused on pathways known to be important for regeneration in vertebrates. To obtain a comprehensive vision of this unique process we have carried out the first de novo transcriptome sequencing for multiple stages of WBR occurring in B. leachi. The identified changes in gene expression during B. leachi WBR offer novel insights into this remarkable ability to regenerate.
resultsThe transcriptome of B. leachi tissue undergoing WBR were analysed using differential gene expression, gene ontology and pathway analyses. We observed up-regulation in the expression of genes involved in wound healing and known developmental pathways including WNT, TGF-β and Notch, during the earliest stages of WBR. Later in WBR, the expression patterns in several pathways required for protein synthesis, biogenesis and the organisation of cellular components were up-regulated.
conclusionsWhile the genes expressed early on are characteristic of a necessary wound healing response to an otherwise lethal injury, the subsequent vast increase in protein synthesis conceivably sustains the reestablishment of the tissue complexity and body axis polarity within the regenerating zooid. We have, for the first time, provided a global overview of the genes and their corresponding pathways that are modulated during WBR in B. leachi.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.