ArticleNature cell biology2016
Extracellular matrix scaffolding guides lumen elongation by inducing anisotropic intercellular mechanical tension.
Article in Nature cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 80 citations in OpenAlex.
- Biophysics of lumen morphogenesis.Development (Cambridge, England) · 2026Review
- Bile canaliculi formation in primary hepatocytes requires α1β1 integrin-dependent adherens junction re-organization.Journal of cell science · 2025Article
- Biomechanical control of vascular morphogenesis by the surrounding stiffness.Nature communications · 2025Article
- Generic comparison of lumen nucleation and fusion in epithelial organoids with and without hydrostatic pressure.Nature communications · 2025Article
- Physics of notochord tube expansion in ascidians.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Dynamic hierarchical ligand anisotropy for competing macrophage regulationBioactive materials · 2025Article
- Interpenetrating networks of fibrillar and amorphous collagen promote cell spreading and hydrogel stability.Acta biomaterialia · 2025Article
- 3D Micropatterned Traction Force Microscopy: A Technique to Control 3D Cell Shape While Measuring Cell-Substrate Force Transmission.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Review
- Designer cellular spheroids with DNA origami for drug screening.Science advances · 2024Article
- Shaping epithelial lumina under pressure.Biochemical Society transactions · 2024Review
- A modular analysis of bile canalicular function and its implications for cholestasis.American journal of physiology. Gastrointestinal and liver physiology · 2023Review
- Hepatocyte apical bulkheads provide a mechanical means to oppose bile pressure.The Journal of cell biology · 2023Article
- The Capillary Morphogenesis Gene 2 Triggers the Intracellular Hallmarks of Collagen VI-Related Muscular Dystrophy.International journal of molecular sciences · 2022Article
- Human iPSC-derived hepatocyte system models cholestasis with tight junction protein 2 deficiency.JHEP reports : innovation in hepatology · 2022Article
- Application of 3D Hepatic Plate-Like Liver Model for Statin-Induced Hepatotoxicity Evaluation.Frontiers in bioengineering and biotechnology · 2022Article
- Organoids.Nature reviews. Methods primers · 2022Article
- Endocytosis in the context-dependent regulation of individual and collective cell properties.Nature reviews. Molecular cell biology · 2021Review
- Modeling alcohol-associated liver disease in a human Liver-Chip.Cell reports · 2021Article
- Cortical tension overrides geometrical cues to orient microtubules in confined protoplasts.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The de novo formation of secretory lumens plays an important role during organogenesis. It involves the establishment of a cellular apical pole and the elongation of luminal cavities. The molecular parameters controlling cell polarization have been heavily scrutinized. In particular, signalling from the extracellular matrix (ECM) proved essential to the proper localization of the apical pole by directed protein transport. However, little is known about the regulation of the shape and the directional development of lumen into tubes. We demonstrate that the spatial scaffolding of cells by ECM can control tube shapes and can direct their elongation. We developed a minimal organ approach comprising of hepatocyte doublets cultured in artificial microniches to precisely control the spatial organization of cellular adhesions in three dimensions. This approach revealed a mechanism by which the spatial repartition of integrin-based adhesion can elicit an anisotropic intercellular mechanical stress guiding the osmotically driven elongation of lumens in the direction of minimal tension. This mechanical guidance accounts for the different morphologies of lumen in various microenvironmental conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.