ArticleNature methods2016
Apollo-NADP(+): a spectrally tunable family of genetically encoded sensors for NADP(+).
Article in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 118 citations in OpenAlex.
- Defining Rational Photoelectron Routing for Targeted Intracellular Energy Transfer.Angewandte Chemie (International ed. in English) · 2026Article
- Effects of aging and anti-aging dietary restriction on regulators of the [NADPH]/[NADPFree radical biology & medicine · 2025Review
- A novel P450 enzyme assay utilizing an NADPBiotechnology letters · 2025Article
- NADPCurrent medicinal chemistry · 2025Review
- A family of NADPH/NADPNature communications · 2024Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Elucidating the spatiotemporal dynamics of glucose metabolism with genetically encoded fluorescent biosensors.Cell reports methods · 2024Review
- Dynamic and Static Regulation of Nicotinamide Adenine Dinucleotide Phosphate: Strategies, Challenges, and Future Directions in Metabolic Engineering.Molecules (Basel, Switzerland) · 2024Review
- A role and mechanism for redox sensing by SENP1 in β-cell responses to high fat feeding.Nature communications · 2024Article
- Measuring the rate of NADPH consumption by glutathione reductase in the cytosol and mitochondria.PloS one · 2024Article
- Oxidized Low-Density Lipoprotein Accumulation Suppresses Glycolysis and Attenuates the Macrophage Inflammatory Response by Diverting Transcription from the HIF-1α to the Nrf2 Pathway.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Apollo-NADPScience advances · 2023Article
- Lighting the light reactions of photosynthesis by means of redox-responsive genetically encoded biosensors for photosynthetic intermediates.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Review
- The pentose phosphate pathway in health and disease.Nature metabolism · 2023Review
- Label-Free Optical Metabolic Imaging in Cells and Tissues.Annual review of biomedical engineering · 2023Review
- NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems.Nature communications · 2023Article
- The GAPDH redox switch safeguards reductive capacity and enables survival of stressed tumour cells.Nature metabolism · 2023Article
- Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease.Cell metabolism · 2023Article
- The multi-faceted role of NADPH in regulating inflammation in activated myeloid cells.Frontiers in immunology · 2023Review
- Fluorescence microscopy imaging of mitochondrial metabolism in cancer cells.Frontiers in oncology · 2023Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
NADPH-dependent antioxidant pathways have a critical role in scavenging hydrogen peroxide (H2O2) produced by oxidative phosphorylation. Inadequate scavenging results in H2O2 accumulation and can cause disease. To measure NADPH/NADP(+) redox states, we explored genetically encoded sensors based on steady-state fluorescence anisotropy due to FRET (fluorescence resonance energy transfer) between homologous fluorescent proteins (homoFRET); we refer to these sensors as Apollo sensors. We created an Apollo sensor for NADP(+) (Apollo-NADP(+)) that exploits NADP(+)-dependent homodimerization of enzymatically inactive glucose-6-phosphate dehydrogenase (G6PD). This sensor is reversible, responsive to glucose-stimulated metabolism and spectrally tunable for compatibility with many other sensors. We used Apollo-NADP(+) to study beta cells responding to oxidative stress and demonstrated that NADPH is significantly depleted before H2O2 accumulation by imaging a Cerulean-tagged version of Apollo-NADP(+) with the H2O2 sensor HyPer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.