ArticleACS sensors2016
Reaction-Based Probes for Imaging Mobile Zinc in Live Cells and Tissues.
Article in ACS sensors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 76 citations in OpenAlex.
- A Reactive and Specific Sensor for Activity-BasedACS sensors · 2024Article
- Zinc-chelating BET bromodomain inhibitors equally target islet endocrine cell types.American journal of physiology. Regulatory, integrative and comparative physiology · 2024Article
- Fluorescent Sensor Based on 1Molecules (Basel, Switzerland) · 2024Article
- Rational design of a genetically encoded NMR zinc sensor.Chemical science · 2023Article
- Advances in reaction-based synthetic fluorescent probes for studying the role of zinc and copper ions in living systems.Journal of clinical biochemistry and nutrition · 2023Article
- Mobile zinc as a modulator of sensory perception.FEBS letters · 2023Review
- Luminescent Metal Complexes as Emerging Tools for Lipid Imaging.Topics in current chemistry (Cham) · 2022Review
- Unusual Reactivity and Metal Affinity of Water-Soluble Dipyrrins.Inorganic chemistry · 2022Article
- Rational Design of Silicon-Based Zinc Ionophores.Angewandte Chemie (International ed. in English) · 2022Article
- Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cells.Life science alliance · 2021Article
- A luminescent Cd(ii) coordination polymer as a multi-responsive fluorescent sensor for ZnRSC advances · 2021Article
- Tools and techniques for illuminating the cell biology of zinc.Biochimica et biophysica acta. Molecular cell research · 2021Review
- Activity-Based Sensing: A Synthetic Methods Approach for Selective Molecular Imaging and Beyond.Angewandte Chemie (International ed. in English) · 2020Review
- Native Zinc Catalyzes Selective and Traceless Release of Small Molecules in β-Cells.Journal of the American Chemical Society · 2020Article
- Diketopyrrolopyrrole-based fluorescence probes for the imaging of lysosomal ZnChemical science · 2019Article
- Turn-on Fluorescence Chemosensor for ZnJournal of fluorescence · 2019Article
- Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes.Molecules (Basel, Switzerland) · 2019Article
- Applications of Nanomaterials Based on Magnetite and Mesoporous Silica on the Selective Detection of Zinc Ion in Live Cell Imaging.Nanomaterials (Basel, Switzerland) · 2018Article
- Report of the International Society for Zinc Biology 5th Meeting, in Collaboration with Zinc-Net (COST Action TD1304)-UCLan Campus, Pyla, Cyprus.International journal of molecular sciences · 2017Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Chelatable, or mobile, forms of zinc play critical signaling roles in numerous biological processes. Elucidating the action of mobile Zn(II) in complex biological environments requires sensitive tools for visualizing, tracking, and manipulating Zn(II) ions. A large toolbox of synthetic photoinduced electron transfer (PET)-based fluorescent Zn(II) sensors are available, but the applicability of many of these probes is limited by poor zinc sensitivity and low dynamic ranges owing to proton interference. We present here a general approach for acetylating PET-based probes containing a variety of fluorophores and zinc-binding units. The new sensors provide substantially improved zinc sensitivity and allow for incubation of live cells and tissue slices with nM probe concentrations, a significant improvement compared to the μM concentrations that are typically required for a measurable fluorescence signal. Acetylation effectively reduces or completely quenches background fluorescence in the metal-free sensor. Binding of Zn(II) selectively and quickly mediates hydrolytic cleavage of the acetyl groups, providing a large fluorescence response. An acetylated blue coumarin-based sensor was used to carry out detailed analyses of metal binding and metal-promoted acetyl hydrolysis. Acetylated benzoresorufin-based red-emitting probes with different zinc-binding sites are effective for sensing Zn(II) ions in live cells when applied at low concentrations (∼50-100 nM). We used green diacetylated Zinpyr1 (DA-ZP1) to image endogenous mobile Zn(II) in the molecular layer of mouse dorsal cochlear nucleus (DCN), confirming that acetylation is a suitable approach for preparing sensors that are highly specific and sensitive to mobile zinc in biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.