ArticleMicrobes and environments2016
FLDS: A Comprehensive dsRNA Sequencing Method for Intracellular RNA Virus Surveillance.
Article in Microbes and environments, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed, 99 citations in OpenAlex.
- Identification of hot spring Obelisk-like RNA replicons and expanded diversity of the Obelisk superfamily.Nature communications · 2026Article
- Identification of a novel toti-like virus symbiotic with Giardia duodenalis.Archives of virology · 2026Article
- The ability of alphavirus replicases to synthesize non-viral type I interferon-inducing RNAs correlates with viral RNA synthesis and has a diverse impact on virus replication and pathogenicity.Journal of virology · 2026Article
- Amarilloviruses of Aquatic Animals.Pathogens (Basel, Switzerland) · 2026Review
- Mycoviruses in Entomopathogenic Fungi.Viruses · 2025Review
- Identification of Obelisk-like covalently closed circular RNA replicon in hot springs by double-stranded RNA sequencing and expansion of the diversity of the Obelisk superfamily.bioRxiv : the preprint server for biology · 2025Article
- Advancing RNA phage biology through meta-omics.Nucleic acids research · 2025Review
- Molecular characterization of a polymycovirus in Leptosphaeria biglobosa.Archives of virology · 2025Article
- "Paraxenoviridae", a putative family of ubiquitous marine bacteriophages with double-stranded RNA genomes.bioRxiv : the preprint server for biology · 2025Article
- Integrated analysis of protein sequence and structure redefines viral diversity and the taxonomy of thebioRxiv : the preprint server for biology · 2025Article
- "Paraxenoviridae", a putative family of globally distributed marine bacteriophages with double-stranded RNA genomes.The ISME journal · 2025Article
- Mycoviruses from Aspergillus fungi involved in fermentation of dried bonito.Virus research · 2024Article
- Metagenomic Analyses of Water Samples of Two Urban Freshwaters in Berlin, Germany, Reveal New Highly Diverse Invertebrate Viruses.Microorganisms · 2024Article
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- New lineages of RNA viruses from clinical isolates ofmSphere · 2024Article
- Metatranscriptomic analysis reveals dissimilarity in viral community activity between an ice-free and ice-covered winter in Lake Erie.mSystems · 2024Article
- Molecular and biological characterization of a novel partitivirus from Talaromyces pinophilus.Virus research · 2024Article
- Double-stranded RNA sequencing reveals distinct riboviruses associated with thermoacidophilic bacteria from hot springs in Japan.Nature microbiology · 2024Article
- DsRNA sequencing revealed a previously missed terminal sequence of a +ssRNA virus that infects dinoflagellate Heterocapsa circularisquama.Virus genes · 2024Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knowledge of the distribution and diversity of RNA viruses is still limited in spite of their possible environmental and epidemiological impacts because RNA virus-specific metagenomic methods have not yet been developed. We herein constructed an effective metagenomic method for RNA viruses by targeting long double-stranded (ds)RNA in cellular organisms, which is a hallmark of infection, or the replication of dsRNA and single-stranded (ss)RNA viruses, except for retroviruses. This novel dsRNA targeting metagenomic method is characterized by an extremely high recovery rate of viral RNA sequences, the retrieval of terminal sequences, and uniform read coverage, which has not previously been reported in other metagenomic methods targeting RNA viruses. This method revealed a previously unidentified viral RNA diversity of more than 20 complete RNA viral genomes including dsRNA and ssRNA viruses associated with an environmental diatom colony. Our approach will be a powerful tool for cataloging RNA viruses associated with organisms of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.