ArticleNature communications2016
GAME9 regulates the biosynthesis of steroidal alkaloids and upstream isoprenoids in the plant mevalonate pathway.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 151 papers.
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Who cites it
151 citing papers in PubMed, 331 citations in OpenAlex.
- Genetic basis of alkaloid divergence in the Solanaceae.Plant molecular biology · 2026Article
- Squalene inPlants (Basel, Switzerland) · 2026Article
- Untargeted metabolomics reveals key steroidal glycoalkaloids and associated metabolites in crude and C18-purified extracts of potato leaves.BMC plant biology · 2026Article
- Unveiling Plant Metabolic Diversity: Integrating Metabolomics with Multi-Omics Approaches for Crop Improvement.Plants (Basel, Switzerland) · 2026Review
- Glycoalkaloid-Free Starch Potatoes Generated by CRISPR/Cas9-Mediated Mutations of Genes in the Glycoalkaloid Biosynthesis Pathway Enable More Sustainable Uses of By-Products From Starch Production.Plant biotechnology journal · 2026Article
- AsAP2 transcriptionally activates ferulate 5-hydroxylase, diverting ferulic acid metabolism toward lignin biosynthesis in Angelica sinensis.Scientific reports · 2025Article
- Decoding steroid-derived metabolite engineering inHorticulture research · 2025Article
- Ontogeny and organ-specific steroidal glycoside diversity is associated with differential expression of steroidal glycoside pathway genes in two Solanum dulcamara leaf chemotypes.Plant biology (Stuttgart, Germany) · 2025Article
- Role of LEAFLESS, an AP2/ERF family transcription factor, in the regulation of trichome specialized metabolism.The New phytologist · 2025Article
- Comparative Transcriptomic Analysis Reveals the Potential Molecular Mechanism Underlying Squalene Biosynthesis in Developing Seeds of Oil-Tea (International journal of molecular sciences · 2025Article
- Genome-wide association study identifies candidate genes for glycoalkaloid biosynthesis in tetraploid potato (Solanum tuberosum L.) tubers.BMC plant biology · 2025Article
- Discovery of Noncanonical Iron and 2-Oxoglutarate Dependent Enzymes Involved in C-C and C-N Bond Formation in Biosynthetic Pathways.ACS bio & med chem Au · 2025Review
- Two reductases complete steroidal glycoalkaloids biosynthesis in potato.The New phytologist · 2025Article
- The AREB transcription factor SaAREB6 promotes drought stress-induced santalol biosynthesis in sandalwood.Horticulture research · 2025Article
- Molecular breeding of tomato: Advances and challenges.Journal of integrative plant biology · 2025Review
- Removal of toxic steroidal glycoalkaloids and bitterness in tomato is controlled by a complex epigenetic and genetic network.Science advances · 2025Article
- A GAME changer in steroidal metabolite biosynthesis.Plant communications · 2025Article
- Transcriptomic and metabolomic profiling of the potato plant response to zebra chip disease.PloS one · 2025Article
- Constitutive expression of full-length or partial ofFrontiers in plant science · 2025Article
- Potato steroidal glycoalkaloids: properties, biosynthesis, regulation and genetic manipulation.Molecular horticulture · 2024Review
91 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Steroidal glycoalkaloids (SGAs) are cholesterol-derived molecules produced by solanaceous species. They contribute to pathogen defence but are toxic to humans and considered as anti-nutritional compounds. Here we show that GLYCOALKALOID METABOLISM 9 (GAME9), an APETALA2/Ethylene Response Factor, related to regulators of alkaloid production in tobacco and Catharanthus roseus, controls SGA biosynthesis. GAME9 knockdown and overexpression in tomato and potato alters expression of SGAs and upstream mevalonate pathway genes including the cholesterol biosynthesis gene STEROL SIDE CHAIN REDUCTASE 2 (SSR2). Levels of SGAs, C24-alkylsterols and the upstream mevalonate and cholesterol pathways intermediates are modified in these plants. Δ(7)-STEROL-C5(6)-DESATURASE (C5-SD) in the hitherto unresolved cholesterol pathway is a direct target of GAME9. Transactivation and promoter-binding assays show that GAME9 exerts its activity either directly or cooperatively with the SlMYC2 transcription factor as in the case of the C5-SD gene promoter. Our findings provide insight into the regulation of SGA biosynthesis and means for manipulating these metabolites in crops.
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