ArticleScientific reports2016
Constructing modular and universal single molecule tension sensor using protein G to study mechano-sensitive receptors.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Imaging and Calibration of Receptor-Specific Cellular Forces Using Biotinylated Ligands.Angewandte Chemie (International ed. in English) · 2026Article
- LFA-1 mechanical dose controls DNAM-1 signalling and perforin polarization in NK cells.RSC advances · 2026Article
- Single Cell Mechanics in Disease Progression.Small science · 2026Review
- Measuring cellular force using DNA-based tension probes: from ensemble to single-molecule studies.Nature protocols · 2026Review
- Contact Dynamics of CytoadheringACS infectious diseases · 2025Article
- DNA Origami Tension Sensors (DOTS) for Single-Molecule Force Measurements at Fluid Intermembrane Junctions.Nano letters · 2025Article
- In silico analyses of molecular force sensors for mechanical characterization of biological systems.Biophysical journal · 2025Article
- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Engineering tunable catch bonds with DNA.Nature communications · 2024Article
- DNA-functionalized artificial mechanoreceptor for de novo force-responsive signaling.Nature chemical biology · 2024Article
- Article
- Unraveling the Dual-Stretch-Mode Impact on Tension Gauge Tethers' Mechanical Stability.Journal of the American Chemical Society · 2024Article
- Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection.Nature biotechnology · 2023Article
- Advanced Mechanical Testing Technologies at the Cellular Level: The Mechanisms and Application in Tissue Engineering.Polymers · 2023Review
- All-Covalent Nuclease-Resistant and Hydrogel-Tethered DNA Hairpin Probes Map pN Cell Traction Forces.ACS applied materials & interfaces · 2023Article
- RAD-TGTs: high-throughput measurement of cellular mechanotype via rupture and delivery of DNA tension probes.Nature communications · 2023Article
- Tension Gauge Tethers as Tension Threshold and Duration Sensors.ACS sensors · 2023Article
- Imaging and detecting intercellular tensile forces in spheroids and embryoid bodies using lipid-modified DNA probes.Frontiers in cell and developmental biology · 2023Article
- Low cost and massively parallel force spectroscopy with fluid loading on a chip.Nature communications · 2022Article
- Facile detection of mechanical forces across proteins in cells with STReTCh.Cell reports methods · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Recently a variety of molecular force sensors have been developed to study cellular forces acting through single mechano-sensitive receptors. A common strategy adopted is to attach ligand molecules on a surface through engineered molecular tethers which report cell-exerted tension on receptor-ligand bonds. This approach generally requires chemical conjugation of the ligand to the force reporting tether which can be time-consuming and labor-intensive. Moreover, ligand-tether conjugation can severely reduce the activity of protein ligands. To address this problem, we developed a Protein G (ProG)-based force sensor in which force-reporting tethers are conjugated to ProG instead of ligands. A recombinant ligand fused with IgG-Fc is conveniently assembled with the force sensor through ProG:Fc binding, therefore avoiding ligand conjugation and purification processes. Using this approach, we determined that molecular tension on E-cadherin is lower than dsDNA unzipping force (nominal value: 12 pN) during initial cadherin-mediated cell adhesion, followed by an escalation to forces higher than 43 pN (nominal value). This approach is highly modular and potentially universal as we demonstrate using two additional receptor-ligand interactions, P-selectin &PSGL-1 and Notch &DLL1.
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