ArticleApplied and environmental microbiology2016
Direct Comparison of Physical Properties of Bacillus subtilis NCIB 3610 and B-1 Biofilms.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Sporulation modulates viscoelastic development through extracellular matrix restructuring inbioRxiv : the preprint server for biology · 2026Article
- Biofilm structure as a key factor in antibiotic tolerance: insights from Bacillus subtilis model systems.NPJ biofilms and microbiomes · 2025Article
- A promiscuous Bcd amino acid dehydrogenase promotes biofilm development in Bacillus subtilis.NPJ biofilms and microbiomes · 2025Article
- Facing Foodborne Pathogen Biofilms with Green Antimicrobial Agents: One Health Approach.Molecules (Basel, Switzerland) · 2025Review
- Resolving spatiotemporal dynamics in bacterial multicellular populations: approaches and challenges.Microbiology and molecular biology reviews : MMBR · 2025Review
- Rapid Stiffness Mapping in Soft Biologic Tissues With Micrometer Resolution Using Optical Multifrequency Time-Harmonic Elastography.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Strain-SpecificInternational journal of nanomedicine · 2025Article
- How bacteria actively use passive physics to make biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The EpiphyteBiomolecules · 2024Article
- Titanium complexes affectRSC medicinal chemistry · 2023Article
- Ultra-lightweight living structural material for enhanced stiffness and environmental sensing.Materials today. Bio · 2023Article
- Experimental challenges in determining the rheological properties of bacterial biofilms.Interface focus · 2022Article
- Probing the growth and mechanical properties ofSynthetic and systems biotechnology · 2022Article
- Competition between growth and shear stress drives intermittency in preferential flow paths in porous medium biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Systems view of Bacillus subtilis pellicle development.NPJ biofilms and microbiomes · 2022Article
- Applications of Antimicrobial Photodynamic Therapy against Bacterial Biofilms.International journal of molecular sciences · 2022Review
- Article
- Bacillus subtilis biofilm formation and social interactions.Nature reviews. Microbiology · 2021Review
- Swarming bacteria undergo localized dynamic phase transition to form stress-induced biofilms.eLife · 2021Article
- The Exo-Polysaccharide Component of Extracellular Matrix is Essential for the Viscoelastic Properties ofInternational journal of molecular sciences · 2020Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many bacteria form surface-attached communities known as biofilms. Due to the extreme resistance of these bacterial biofilms to antibiotics and mechanical stresses, biofilms are of growing interest not only in microbiology but also in medicine and industry. Previous studies have determined the extracellular polymeric substances present in the matrix of biofilms formed by Bacillus subtilis NCIB 3610. However, studies on the physical properties of biofilms formed by this strain are just emerging. In particular, quantitative data on the contributions of biofilm matrix biopolymers to these physical properties are lacking. Here, we quantitatively investigated three physical properties of B. subtilis NCIB 3610 biofilms: the surface roughness and stiffness and the bulk viscoelasticity of these biofilms. We show how specific biomolecules constituting the biofilm matrix formed by this strain contribute to those biofilm properties. In particular, we demonstrate that the surface roughness and surface elasticity of 1-day-old NCIB 3610 biofilms are strongly affected by the surface layer protein BslA. For a second strain,B. subtilis B-1, which forms biofilms containing mainly γ-polyglutamate, we found significantly different physical biofilm properties that are also differently affected by the commonly used antibacterial agent ethanol. We show that B-1 biofilms are protected from ethanol-induced changes in the biofilm's stiffness and that this protective effect can be transferred to NCIB 3610 biofilms by the sole addition of γ-polyglutamate to growing NCIB 3610 biofilms. Together, our results demonstrate the importance of specific biofilm matrix components for the distinct physical properties of B. subtilis biofilms.
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