ArticleDevelopmental biology2016
Mouse sperm begin to undergo acrosomal exocytosis in the upper isthmus of the oviduct.
Article in Developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- Restoration of Motility in Acrosome-Reacted Boar Spermatozoa Induced by the Ionophore 4-Br-A23187.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Efficacy and safety evaluation of HyperSperm treatment in human semen samples.Journal of translational medicine · 2026Article
- From reservoir to rendezvous: the journey of sperm through the oviduct.Reproduction, fertility, and development · 2026Review
- Sperm-female interactions in the pig oviduct, a key for insemination success?The Journal of reproduction and development · 2026Review
- Let the female tract choose: finding the sperm to enhanceAnimal reproduction · 2026Article
- Dynamic Remodeling of the Zona Pellucida: Implications for Oocyte Competence and Assisted Reproduction.International journal of molecular sciences · 2025Review
- D-Mannose-Mediated metabolic pathways sustain the molecular signatures of sperm function and fertilization.Journal of advanced research · 2025Article
- Article
- Influences of 5-hydroxytriptamine on sperm hyperactivation and in vitro fertility in rats.The Journal of reproduction and development · 2025Article
- Mating Increases CHST10 Activity in Rat Oviductal Mucosa to Induce the Synthesis of HNK-1 Glycoproteins: Possible Role in Sperm-Oviduct Interactions.International journal of molecular sciences · 2025Article
- Dynamics of post-ejaculated intrauterine environment in mice.microPublication biology · 2025Article
- Immunolocalization and proteomic analyses of IZUMO1 in porcine spermatozoa.Frontiers in cell and developmental biology · 2025Article
- Increased reproductive outcomes after optimized sperm preparation.Frontiers in cell and developmental biology · 2025Article
- Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization.eLife · 2024Article
- Sperm in the Mammalian Female Reproductive Tract: Surfing Through the Tract to Try to Beat the Odds.Annual review of animal biosciences · 2024Review
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- Article
- Membrane potential hyperpolarization: a critical factor in acrosomal exocytosis and fertilization in sperm within the female reproductive tract.Frontiers in cell and developmental biology · 2024Article
- Mechanisms That Protect Mammalian Sperm from the Spontaneous Acrosome Reaction.International journal of molecular sciences · 2023Review
- Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies†.Biology of reproduction · 2023Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
Abstract
Recent evidence demonstrated that most fertilizing mouse sperm undergo acrosomal exocytosis (AE) before binding to the zona pellucida of the eggs. However, the sites where fertilizing sperm could initiate AE and what stimuli trigger it remain unknown. Therefore, the aim of this study was to determine physiological sites of AE by using double transgenic mouse sperm, which carried EGFP in the acrosome and DsRed2 fluorescence in mitochondria. Using live imaging of sperm during in vitro fertilization of cumulus-oocyte complexes, it was observed that most sperm did not undergo AE. Thus, the occurrence of AE within the female reproductive tract was evaluated in the physiological context where this process occurs. Most sperm in the lower segments of the oviduct were acrosome-intact; however, a significant number of sperm that reached the upper isthmus had undergone AE. In the ampulla, only 5% of the sperm were acrosome-intact. These results support our previous observations that most of mouse sperm do not initiate AE close to or on the ZP, and further demonstrate that a significant proportion of sperm initiate AE in the upper segments of the oviductal isthmus.
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