ArticlePloS one2016
Temporal Gene Expression Analysis and RNA Silencing of Single and Multiple Members of Gene Family in the Lone Star Tick Amblyomma americanum.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Reversible Cationic Glycopolymer Hydrogels for RNA Delivery in Ticks.ACS polymers Au · 2026Article
- Multi-Omics Technologies Applied to Improve Tick Research.Microorganisms · 2025Review
- Identification and knockdown effect of disulfide isomerase in the Haemaphysalis longicornis (Acari: Ixodidae).Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2025Article
- Glycine rich proteins of ticks: more than a cement component.Parasitology · 2024Review
- Effect of Silencing subolesin and enolase impairs gene expression, engorgement and reproduction inJournal of veterinary science · 2024Article
- Article
- Cationic Glycopolyelectrolytes for RNA Interference in Tick Cells.Biomacromolecules · 2022Article
- Alpha-Gal Syndrome: Involvement ofFrontiers in cellular and infection microbiology · 2021Article
- Repurposing of Glycine-Rich Proteins in Abiotic and Biotic Stresses in the Lone-Star Tick (Frontiers in physiology · 2019Article
- The transcriptome of the lone star tick,Journal of Asia-Pacific entomology · 2018Article
- Article
- De novo assembly and annotation of Hyalomma dromedarii tick (Acari: Ixodidae) sialotranscriptome with regard to gender differences in gene expression.Parasites & vectors · 2018Article
- Tick attachment cement - reviewing the mysteries of a biological skin plug system.Biological reviews of the Cambridge Philosophical Society · 2018Review
- Article
- Identification and characterization of proteins in the Amblyomma americanum tick cement cone.International journal for parasitology · 2018Article
- Disruption of blood meal-responsive serpins prevents Ixodes scapularis from feeding to repletion.Ticks and tick-borne diseases · 2018Article
- Amblyomma maculatum SECIS binding protein 2 and putative selenoprotein P are indispensable for pathogen replication and tick fecundity.Insect biochemistry and molecular biology · 2017Article
- Sialotranscriptomics of Rhipicephalus zambeziensis reveals intricate expression profiles of secretory proteins and suggests tight temporal transcriptional regulation during blood-feeding.Parasites & vectors · 2017Article
- Analysis of the Salivary Gland Transcriptome of Unfed and Partially FedFrontiers in cellular and infection microbiology · 2017Article
- Rickettsia parkeri colonization in Amblyomma maculatum: the role of superoxide dismutases.Parasites & vectors · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Saliva is an integral factor in the feeding success of veterinary and medically important ticks. Therefore, the characterization of the proteins present in tick saliva is an important area of tick research. Here, we confirmed previously generated sialotranscriptome data using quantitative real-time PCR. The information obtained in this in-depth study of gene expression was used to measure the effects of metalloprotease gene silencing on tick feeding. We analyzed the temporal expression of seven housekeeping genes and 44 differentially expressed salivary molecules selected from a previously published Amblyomma americanum sialotranscriptome. Separate reference genes were selected for the salivary glands and midgut from among the seven housekeeping genes, to normalize the transcriptional expression of differentially expressed genes. The salivary gland reference gene, ubiquitin, was used to normalize the expression of 44 salivary genes. Unsurprisingly, each gene family was expressed throughout the blood meal, but the expression of specific genes differed at each time point. To further clarify the complex nature of the many proteins found in the saliva, we disrupted the translation of several members of the metalloprotease family. Intriguingly, the nucleotide sequence similarity of the reprolysin metalloprotease gene family is so homologous that a single synthesized dsRNA sequence knocked down multiple members of the family. The use of multigene knockdown yielded a more significant picture of the role of metalloproteases in tick feeding success, and changes were observed in the female engorgement weight and larval hatching success. Interestingly, the depletion of metalloprotease transcripts also reduced the total number of bacteria present in the salivary glands. These data provide insight into the expression and functions of tick salivary proteins expressed while feeding on its host.
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