ArticleThe ISME journal2016
Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment.
Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 65 citations in OpenAlex.
- Biodegradation of synthetic organic pollutants: principles, progress, problems, and perspectives.FEMS microbiology reviews · 2025Review
- Ring A Cleaving Beta-Diketone Hydrolase Is a Key Enzyme of Steroid Degradation in Anaerobic Bacteria.Environmental microbiology · 2025Article
- The microbiota: a key regulator of health, productivity, and reproductive success in mammals.Frontiers in microbiology · 2024Review
- Deciphering chloramphenicol biotransformation mechanisms and microbial interactions via integrated multi-omics and cultivation-dependent approaches.Microbiome · 2022Article
- Medicines as an emergent contaminant: the review of microbial biodegration potential.Folia microbiologica · 2022Review
- Degradation of Bile Acids by Soil and Water Bacteria.Microorganisms · 2021Review
- Article
- Specialized metabolic functions of keystone taxa sustain soil microbiome stability.Microbiome · 2021Article
- Suitability of Immobilized Systems for Microbiological Degradation of Endocrine Disrupting Compounds.Molecules (Basel, Switzerland) · 2020Review
- Microbial degradation of steroid sex hormones: implications for environmental and ecological studies.Microbial biotechnology · 2020Review
- Retroconversion of estrogens into androgens by bacteria via a cobalamin-mediated methylation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- Metabolites Involved in Aerobic Degradation of the A and B Rings of Estrogen.Applied and environmental microbiology · 2019Article
- Bacterial steroid hydroxylases: enzyme classes, their functions and comparison of their catalytic mechanisms.Applied microbiology and biotechnology · 2018Review
- Article
- Estrogen Degraders and Estrogen Degradation Pathway Identified in an Activated Sludge.Applied and environmental microbiology · 2018Article
- Article
- Catabolism of the Last Two Steroid Rings inmBio · 2017Article
- A Community Multi-Omics Approach towards the Assessment of Surface Water Quality in an Urban River System.International journal of environmental research and public health · 2017Article
- Biochemical Mechanisms and Microorganisms Involved in Anaerobic Testosterone Metabolism in Estuarine Sediments.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Steroid hormones, such as androgens, are common surface-water contaminants. However, literature on the ecophysiological relevance of steroid-degrading organisms in the environment, particularly in anoxic ecosystems, is extremely limited. We previously reported that Steroidobacter denitrificans anaerobically degrades androgens through the 2,3-seco pathway. In this study, the genome of Sdo. denitrificans was completely sequenced. Transcriptomic data revealed gene clusters that were distinctly expressed during anaerobic growth on testosterone. We isolated and characterized the bifunctional 1-testosterone hydratase/dehydrogenase, which is essential for anaerobic degradation of steroid A-ring. Because of apparent substrate preference of this molybdoenzyme, corresponding genes, along with the signature metabolites of the 2,3-seco pathway, were used as biomarkers to investigate androgen biodegradation in the largest sewage treatment plant in Taipei, Taiwan. Androgen metabolite analysis indicated that denitrifying bacteria in anoxic sewage use the 2,3-seco pathway to degrade androgens. Metagenomic analysis and PCR-based functional assays showed androgen degradation in anoxic sewage by Thauera spp. through the action of 1-testosterone hydratase/dehydrogenase. Our integrative 'omics' approach can be used for culture-independent investigations of the microbial degradation of structurally complex compounds where isotope-labeled substrates are not easily available.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.