ArticleThe ISME journal2016
Coupled RNA-SIP and metatranscriptomics of active chemolithoautotrophic communities at a deep-sea hydrothermal vent.
Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 103 citations in OpenAlex.
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- Metabolic and population profiles of active subseafloor autotrophs in young oceanic crust at deep-sea hydrothermal vents.Applied and environmental microbiology · 2025Article
- The chemosynthetic biofilm microbiome of deep-sea hydrothermal vents across space and time.Environmental microbiome · 2025Article
- Intraspecific divergence withinProceedings. Biological sciences · 2025Article
- Metagenomic analysis of deep-sea bacterial communities in the Makassar and Lombok Straits.Scientific reports · 2024Article
- Salinity affects microbial function genes related to nutrient cycling in arid regions.Frontiers in microbiology · 2024Article
- Strategies of chemolithoautotrophs adapting to high temperature and extremely acidic conditions in a shallow hydrothermal ecosystem.Microbiome · 2023Article
- Microbial gene expression in Guaymas Basin subsurface sediments responds to hydrothermal stress and energy limitation.The ISME journal · 2023Article
- Advances and perspectives of using stable isotope probing (SIP)-based technologies in contaminant biodegradation.Water research X · 2023Article
- DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Impact of salinity origin on microbial communities in saline springs within the Illinois Basin, USA.Environmental microbiology · 2022Article
- Biodiversity and degradation potential of oil-degrading bacteria isolated from sediments of hydrothermal and non-hydrothermal areas of the Southwest Mid-Indian Ocean Ridge.Environmental science and pollution research international · 2022Article
- Genus-Specific Carbon Fixation Activity Measurements Reveal Distinct Responses to Oxygen among Hydrothermal VentApplied and environmental microbiology · 2022Article
- Quantifying the effects of hydrogen on carbon assimilation in a seafloor microbial community associated with ultramafic rocks.The ISME journal · 2022Article
- An overview of experimental simulations of microbial activity in early Earth.Frontiers in microbiology · 2022Review
- Depth wide distribution and metabolic potential of chemolithoautotrophic microorganisms reactivated from deep continental granitic crust underneath the Deccan Traps at Koyna, India.Frontiers in microbiology · 2022Article
- Approaches to Unmask Functioning of the Uncultured Microbial Majority From Extreme Habitats on the Seafloor.Frontiers in microbiology · 2022Review
- Evolution of Thermophilic Microbial Communities from a Deep-Sea Hydrothermal Chimney under Electrolithoautotrophic Conditions with Nitrate.Microorganisms · 2021Article
- Metabolic Features of Ganjang (a Korean Traditional Soy Sauce) Fermentation Revealed by Genome-Centered Metatranscriptomics.mSystems · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chemolithoautotrophic microbial community of the rocky subseafloor potentially provides a large amount of organic carbon to the deep ocean, yet our understanding of the activity and metabolic complexity of subseafloor organisms remains poorly described. A combination of metagenomic, metatranscriptomic, and RNA stable isotope probing (RNA-SIP) analyses were used to identify the metabolic potential, expression patterns, and active autotrophic bacteria and archaea and their pathways present in low-temperature hydrothermal fluids from Axial Seamount, an active submarine volcano. Metagenomic and metatranscriptomic results showed the presence of genes and transcripts for sulfur, hydrogen, and ammonium oxidation, oxygen respiration, denitrification, and methanogenesis, as well as multiple carbon fixation pathways. In RNA-SIP experiments across a range of temperatures under reducing conditions, the enriched (13)C fractions showed differences in taxonomic and functional diversity. At 30 °C and 55 °C, Epsilonproteobacteria were dominant, oxidizing hydrogen and primarily reducing nitrate. Methanogenic archaea were also present at 55 °C, and were the only autotrophs present at 80 °C. Correspondingly, the predominant CO2 fixation pathways changed from the reductive tricarboxylic acid (rTCA) cycle to the reductive acetyl-CoA pathway with increasing temperature. By coupling RNA-SIP with meta-omics, this study demonstrates the presence and activity of distinct chemolithoautotrophic communities across a thermal gradient of a deep-sea hydrothermal vent.
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