Evidence map›Paper›PMID 26871860›Full record

ArticleTissue engineering. Part A2016

Adipose-Derived Stem Cell-Seeded Hydrogels Increase Endogenous Progenitor Cell Recruitment and Neovascularization in Wounds.

Revanth Kosaraju, Robert C Rennert, Zeshaan N Maan, Dominik Duscher, Janos Barrera, Alexander J Whittam, Michael Januszyk, Jayakumar Rajadas, Melanie Rodrigues, Geoffrey C Gurtner

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering. Part A, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 76 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Revanth Kosaraju1 Department of Surgery, Stanford University School of Medicine , Stanford, California.
Robert C Rennert1 Department of Surgery, Stanford University School of Medicine , Stanford, California.
Zeshaan N Maan1 Department of Surgery, Stanford University School of Medicine , Stanford, California.
Dominik Duscher1 Department of Surgery, Stanford University School of Medicine , Stanford, California.
Janos Barrera1 Department of Surgery, Stanford University School of Medicine , Stanford, California.
Alexander J Whittam1 Department of Surgery, Stanford University School of Medicine , Stanford, California.
Michael Januszyk1 Department of Surgery, Stanford University School of Medicine , Stanford, California.
Jayakumar Rajadas3 Biomaterials and Advanced Drug Delivery Center, Stanford University , Stanford, California.
Melanie Rodrigues1 Department of Surgery, Stanford University School of Medicine , Stanford, California.
Geoffrey C Gurtner1 Department of Surgery, Stanford University School of Medicine , Stanford, California.
Stanford University · US

Funding

Reversal of Progenitor Cell Deficits in DiabetesR01DK074095 · NIDDK · UNIVERSITY OF ARIZONA · PI GURTNER, GEOFFREY C · 2005 to 2025
$6.4M
Targeting HIF-1 alpha Dysfunction in Complications of AgingR01AG025016 · NIA · STANFORD UNIVERSITY · PI GURTNER, GEOFFREY C · 2004 to 2015
$3.5M
NIA NIH HHS R01 AG025016NIDDK NIH HHS R01 DK074095
6 · The paper itself

Abstract

Adipose-derived mesenchymal stem cells (ASCs) are appealing for cell-based wound therapies because of their accessibility and ease of harvest, but their utility is limited by poor cell survival within the harsh wound microenvironment. In prior work, our laboratory has demonstrated that seeding ASCs within a soft pullulan-collagen hydrogel enhances ASC survival and improves wound healing. To more fully understand the mechanism of this therapy, we examined whether ASC-seeded hydrogels were able to modulate the recruitment and/or functionality of endogenous progenitor cells. Employing a parabiosis model and fluorescence-activated cell sorting analysis, we demonstrate that application of ASC-seeded hydrogels to wounds, when compared with injected ASCs or a noncell control, increased the recruitment of provascular circulating bone marrow-derived mesenchymal progenitor cells (BM-MPCs). BM-MPCs comprised 23.0% of recruited circulating progenitor cells in wounds treated with ASC-seeded hydrogels versus 8.4% and 2.1% in those treated with controls, p < 0.05. Exploring the potential for functional modulation of BM-MPCs, we demonstrate a statistically significant increase in BM-MPC migration, proliferation, and tubulization when exposed to hydrogel-seeded ASC-conditioned medium versus control ASC-conditioned medium (73.8% vs. 51.4% scratch assay closure; 9.1% vs. 1.4% proliferation rate; 10.2 vs. 5.5 tubules/HPF; p < 0.05 for all assays). BM-MPC expression of genes related to cell stemness and angiogenesis was also significantly increased following exposure to hydrogel-seeded ASC-conditioned medium (p < 0.05). These data suggest that ASC-seeded hydrogels improve both progenitor cell recruitment and functionality to effect greater neovascularization.

Indexed as

Neovascularization, PhysiologicAdipose TissueAnimalsHydrogelsMesenchymal Stem CellsMiceWounds and InjuriesHydrogels

Identifiers

PMID26871860
PMCPMC4779321
OpenAlexW2237176023

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.