ArticlePloS one2016
Testicular Busulfan Injection in Mice to Prepare Recipients for Spermatogonial Stem Cell Transplantation Is Safe and Non-Toxic.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 46 citations in OpenAlex.
- Germ cell depletion using HSV-TK in mouse testes.Scientific reports · 2025Article
- Effect of IL-1β on the Development of Spermatogenesis In Vitro in Normal and Busulfan-Treated Immature Mice.International journal of molecular sciences · 2024Article
- Effect of N-acetyl-L-cysteine on Testicular Tissue in Busulfan-Induced Dysfunction in the Male Reproductive System.The world journal of men's health · 2023Article
- Melatonin in cryopreservation media improves transplantation efficiency of frozen-thawed spermatogonial stem cells into testes of azoospermic mice.Stem cell research & therapy · 2022Article
- Intratesticular versus intraperitoneal injection of Busulfan for the induction of azoospermia in a rat model.BMC pharmacology & toxicology · 2022Article
- Characterization and Survival of Human Infant Testicular Cells After Direct Xenotransplantation.Frontiers in endocrinology · 2022Article
- Article
- Spermatogonial stem cell survival in ram lambs following busulfan treatment.Animal reproduction · 2020Article
- Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys.International journal of molecular sciences · 2019Article
- Progress on the study of the mechanism of busulfan cytotoxicity.Cytotechnology · 2018Review
- Long-term health in recipients of transplanted in vitro propagated spermatogonial stem cells.Human reproduction (Oxford, England) · 2018Article
- An efficient method for generating a germ cell depleted animal model for studies related to spermatogonial stem cell transplantation.Stem cell research & therapy · 2016Article
- Establishing a nonlethal and efficient mouse model of male gonadotoxicity by intraperitoneal busulfan injection.Asian journal of andrologyArticle
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current methods of administering busulfan to remove the endogenous germ cells cause hematopoietic toxicity, require special instruments and a narrow transplantation time. We use a direct testicular injection of busulfan method for preparing recipients for SSC transplantation. Male ICR mice (recipients) were divided into four groups, and two experimental groups were treated with a bilateral testicular injection of 4 or 6 mg/kg/side busulfan (n = 60 per concentration group). Mice received an intraperitoneal injection (i.p.) of 40 mg/kg busulfan (n = 60, positive control) and bilateral testicular injections of 50% DMSO (n = 60, negative control). Donor SSCs from RFP-transgenic C57BL/6J mice were introduced into the seminiferous tubules of each recipient testis via efferent duct injection on day 16-17 after busulfan treatment. Recipient mice mated with mature female ICR mice and the number of progeny was recorded. The index detected at day 14, 21, 28, 35 and 70 after busulfan treatment. Blood analysis shows that the toxicity of busulfan treated groups was much lower than i.p. injection groups. Fertility was restored in mice treated with busulfan and donor-derived offspring were obtained after SSC transplantation. Our study indicated that intratesticular injection busulfan for the preparation of recipients in mice is safe and feasible.
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