ArticlePLoS neglected tropical diseases2016
Sensitive Detection of Plasmodium vivax Using a High-Throughput, Colourimetric Loop Mediated Isothermal Amplification (HtLAMP) Platform: A Potential Novel Tool for Malaria Elimination.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 4 of them syntheses that pooled it.
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Who cites it
30 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Loop-mediated isothermal amplification (LAMP) test in the detection of uncomplicated malaria in pregnancy: a meta-analysis of diagnostic accuracy.Malaria journal · 2022Pooled it
- Malaria distribution and performance of malaria diagnostic methods in Malaysia (1980-2019): a systematic review.Malaria journal · 2020Pooled it
- Rapid diagnostic tests for Plasmodium vivax malaria in endemic countries.The Cochrane database of systematic reviews · 2020Pooled it
- Loop-mediated isothermal amplification (LAMP) test for diagnosis of uncomplicated malaria in endemic areas: a meta-analysis of diagnostic test accuracy.Malaria journal · 2020Pooled it
- Review
- Recombinase-Aided Loop-Mediated Isothermal Amplification on Human Plasmodium knowlesi.The American journal of tropical medicine and hygiene · 2024Article
- Malaria diagnosis using a combined system of a simple and fast extraction method with a lyophilised Dual-LAMP assay in a non-endemic setting.Pathogens and global health · 2024Article
- Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?Malaria journal · 2023Article
- Sensitive Detection of Asymptomatic and Symptomatic Malaria with Seven Novel Parasite-Specific LAMP Assays and Translation for Use at Point-of-Care.Microbiology spectrum · 2023Article
- Sample-to-Answer Microfluidic Nucleic Acid Testing (NAT) on Lab-on-a-Disc for Malaria Detection at Point of Need.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Development of Two-Tube Loop-Mediated Isothermal Amplification Assay for Differential Diagnosis ofDiagnostics (Basel, Switzerland) · 2021Article
- Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP.Malaria journal · 2021Article
- Validation of SYBR green I based closed-tube loop-mediated isothermal amplification (LAMP) assay for diagnosis of knowlesi malaria.Malaria journal · 2021Article
- Loop-mediated isothermal amplification (LAMP) assay for the diagnosis of imported malaria: a narrative review.Le infezioni in medicina · 2021Review
- Diagnostic Methods for Non-Falciparum Malaria.Frontiers in cellular and infection microbiology · 2021Review
- An Ultra-Sensitive Technique: UsingPathogens (Basel, Switzerland) · 2020Article
- Implications of asymptomatic infection for the natural history of selected parasitic tropical diseases.Seminars in immunopathology · 2020Review
- Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar.Malaria journal · 2020Article
- Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria.Memorias do Instituto Oswaldo Cruz · 2019Article
- Poor Diagnostic Performance of a Species-Specific Loop-Mediated Isothermal Amplification (LAMP) Platform for Malaria.European journal of microbiology & immunology · 2018Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
introductionPlasmodium vivax malaria has a wide geographic distribution and poses challenges to malaria elimination that are likely to be greater than those of P. falciparum. Diagnostic tools for P. vivax infection in non-reference laboratory settings are limited to microscopy and rapid diagnostic tests but these are unreliable at low parasitemia. The development and validation of a high-throughput and sensitive assay for P. vivax is a priority.
methodsA high-throughput LAMP assay targeting a P. vivax mitochondrial gene and deploying colorimetric detection in a 96-well plate format was developed and evaluated in the laboratory. Diagnostic accuracy was compared against microscopy, antigen detection tests and PCR and validated in samples from malaria patients and community controls in a district hospital setting in Sabah, Malaysia.
resultsThe high throughput LAMP-P. vivax assay (HtLAMP-Pv) performed with an estimated limit of detection of 1.4 parasites/ μL. Assay primers demonstrated cross-reactivity with P. knowlesi but not with other Plasmodium spp. Field testing of HtLAMP-Pv was conducted using 149 samples from symptomatic malaria patients (64 P. vivax, 17 P. falciparum, 56 P. knowlesi, 7 P. malariae, 1 mixed P. knowlesi/P. vivax, with 4 excluded). When compared against multiplex PCR, HtLAMP-Pv demonstrated a sensitivity for P. vivax of 95% (95% CI 87-99%); 61/64), and specificity of 100% (95% CI 86-100%); 25/25) when P. knowlesi samples were excluded. HtLAMP-Pv testing of 112 samples from asymptomatic community controls, 7 of which had submicroscopic P. vivax infections by PCR, showed a sensitivity of 71% (95% CI 29-96%; 5/7) and specificity of 93% (95% CI87-97%; 98/105).
conclusionThis novel HtLAMP-P. vivax assay has the potential to be a useful field applicable molecular diagnostic test for P. vivax infection in elimination settings.
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