Developing Michigan Cancer Foundation 7 Cells with Stable Expression of E7 Gene of Human Papillomavirus Type 16.

Faezeh Ghasemi, Sina Rostami, Maryam Sadat Nabavinia, Zahra Meshkat

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Article in Iranian journal of pathology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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4 authors at 2 institutions in 1 country.

Faezeh GhasemiDept. of New Sciences and Technology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Sina RostamiDept. of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.

Maryam Sadat NabaviniaDept. of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

Zahra MeshkatAntimicrobial Resistance Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Mashhad University of Medical Sciences · IRFerdowsi University of Mashhad · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman papillomavirus (HPV) is responsible for the development of cervical neoplasia. Infection with human papillomavirus type 16 (HPV-16) is a major risk factor for the development of cervical cancer. The virus encodes three oncoproteins (E5, E6 and E7), of which, the E7 oncoprotein is the major protein involved in cell immortalization and transformation of the infected cells. The aim of the current study was to develop Michigan Cancer Foundation 7 (MCF7) cells, which could stably express E7 protein of HPV type 16.

methodsE7 gene of HPV type 16 was introduced into MCF7 cells by Lipofectamine 2000 reagent and the transfected cells were treated with G418 antibiotic. After antibiotic selection of the transfected cells, stable expression of E7 gene of HPV16 was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR).

resultsAntibiotic selections of transfected cells were performed and transfected cells were alive in cytotoxic concentration of the antibiotic. RNA was extracted from transfected cells and E7 gene of HPV16 was amplified by RT-PCR method and a 350-bp band corresponds to E7 was observed.

conclusionResults confirmed the stable transfection of cells. The stably transfected cells can be used as a useful tool in future studies on HPV16 and cancers caused by this virus.

Indexed as

E7 geneHPV-16MCF7RT-PCRstable expression

Identifiers

PMID26870142

PMCPMC4749194

OpenAlexW2261967964

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